How to Write a Conclusion for Research Papers (with Examples)

How to Write a Conclusion for Research Papers (with Examples)

The conclusion of a research paper is a crucial section that plays a significant role in the overall impact and effectiveness of your research paper. However, this is also the section that typically receives less attention compared to the introduction and the body of the paper. The conclusion serves to provide a concise summary of the key findings, their significance, their implications, and a sense of closure to the study. Discussing how can the findings be applied in real-world scenarios or inform policy, practice, or decision-making is especially valuable to practitioners and policymakers. The research paper conclusion also provides researchers with clear insights and valuable information for their own work, which they can then build on and contribute to the advancement of knowledge in the field.

The research paper conclusion should explain the significance of your findings within the broader context of your field. It restates how your results contribute to the existing body of knowledge and whether they confirm or challenge existing theories or hypotheses. Also, by identifying unanswered questions or areas requiring further investigation, your awareness of the broader research landscape can be demonstrated.

Remember to tailor the research paper conclusion to the specific needs and interests of your intended audience, which may include researchers, practitioners, policymakers, or a combination of these.

Table of Contents

What is a conclusion in a research paper, summarizing conclusion, editorial conclusion, externalizing conclusion, importance of a good research paper conclusion, how to write a conclusion for your research paper, research paper conclusion examples.

  • How to write a research paper conclusion with Paperpal? 

Frequently Asked Questions

A conclusion in a research paper is the final section where you summarize and wrap up your research, presenting the key findings and insights derived from your study. The research paper conclusion is not the place to introduce new information or data that was not discussed in the main body of the paper. When working on how to conclude a research paper, remember to stick to summarizing and interpreting existing content. The research paper conclusion serves the following purposes: 1

  • Warn readers of the possible consequences of not attending to the problem.
  • Recommend specific course(s) of action.
  • Restate key ideas to drive home the ultimate point of your research paper.
  • Provide a “take-home” message that you want the readers to remember about your study.

sample conclusion on research

Types of conclusions for research papers

In research papers, the conclusion provides closure to the reader. The type of research paper conclusion you choose depends on the nature of your study, your goals, and your target audience. I provide you with three common types of conclusions:

A summarizing conclusion is the most common type of conclusion in research papers. It involves summarizing the main points, reiterating the research question, and restating the significance of the findings. This common type of research paper conclusion is used across different disciplines.

An editorial conclusion is less common but can be used in research papers that are focused on proposing or advocating for a particular viewpoint or policy. It involves presenting a strong editorial or opinion based on the research findings and offering recommendations or calls to action.

An externalizing conclusion is a type of conclusion that extends the research beyond the scope of the paper by suggesting potential future research directions or discussing the broader implications of the findings. This type of conclusion is often used in more theoretical or exploratory research papers.

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The conclusion in a research paper serves several important purposes:

  • Offers Implications and Recommendations : Your research paper conclusion is an excellent place to discuss the broader implications of your research and suggest potential areas for further study. It’s also an opportunity to offer practical recommendations based on your findings.
  • Provides Closure : A good research paper conclusion provides a sense of closure to your paper. It should leave the reader with a feeling that they have reached the end of a well-structured and thought-provoking research project.
  • Leaves a Lasting Impression : Writing a well-crafted research paper conclusion leaves a lasting impression on your readers. It’s your final opportunity to leave them with a new idea, a call to action, or a memorable quote.

sample conclusion on research

Writing a strong conclusion for your research paper is essential to leave a lasting impression on your readers. Here’s a step-by-step process to help you create and know what to put in the conclusion of a research paper: 2

  • Research Statement : Begin your research paper conclusion by restating your research statement. This reminds the reader of the main point you’ve been trying to prove throughout your paper. Keep it concise and clear.
  • Key Points : Summarize the main arguments and key points you’ve made in your paper. Avoid introducing new information in the research paper conclusion. Instead, provide a concise overview of what you’ve discussed in the body of your paper.
  • Address the Research Questions : If your research paper is based on specific research questions or hypotheses, briefly address whether you’ve answered them or achieved your research goals. Discuss the significance of your findings in this context.
  • Significance : Highlight the importance of your research and its relevance in the broader context. Explain why your findings matter and how they contribute to the existing knowledge in your field.
  • Implications : Explore the practical or theoretical implications of your research. How might your findings impact future research, policy, or real-world applications? Consider the “so what?” question.
  • Future Research : Offer suggestions for future research in your area. What questions or aspects remain unanswered or warrant further investigation? This shows that your work opens the door for future exploration.
  • Closing Thought : Conclude your research paper conclusion with a thought-provoking or memorable statement. This can leave a lasting impression on your readers and wrap up your paper effectively. Avoid introducing new information or arguments here.
  • Proofread and Revise : Carefully proofread your conclusion for grammar, spelling, and clarity. Ensure that your ideas flow smoothly and that your conclusion is coherent and well-structured.

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Remember that a well-crafted research paper conclusion is a reflection of the strength of your research and your ability to communicate its significance effectively. It should leave a lasting impression on your readers and tie together all the threads of your paper. Now you know how to start the conclusion of a research paper and what elements to include to make it impactful, let’s look at a research paper conclusion sample.

sample conclusion on research

How to write a research paper conclusion with Paperpal?

A research paper conclusion is not just a summary of your study, but a synthesis of the key findings that ties the research together and places it in a broader context. A research paper conclusion should be concise, typically around one paragraph in length. However, some complex topics may require a longer conclusion to ensure the reader is left with a clear understanding of the study’s significance. Paperpal, an AI writing assistant trusted by over 800,000 academics globally, can help you write a well-structured conclusion for your research paper. 

  • Sign Up or Log In: Create a new Paperpal account or login with your details.  
  • Navigate to Features : Once logged in, head over to the features’ side navigation pane. Click on Templates and you’ll find a suite of generative AI features to help you write better, faster.  
  • Generate an outline: Under Templates, select ‘Outlines’. Choose ‘Research article’ as your document type.  
  • Select your section: Since you’re focusing on the conclusion, select this section when prompted.  
  • Choose your field of study: Identifying your field of study allows Paperpal to provide more targeted suggestions, ensuring the relevance of your conclusion to your specific area of research. 
  • Provide a brief description of your study: Enter details about your research topic and findings. This information helps Paperpal generate a tailored outline that aligns with your paper’s content. 
  • Generate the conclusion outline: After entering all necessary details, click on ‘generate’. Paperpal will then create a structured outline for your conclusion, to help you start writing and build upon the outline.  
  • Write your conclusion: Use the generated outline to build your conclusion. The outline serves as a guide, ensuring you cover all critical aspects of a strong conclusion, from summarizing key findings to highlighting the research’s implications. 
  • Refine and enhance: Paperpal’s ‘Make Academic’ feature can be particularly useful in the final stages. Select any paragraph of your conclusion and use this feature to elevate the academic tone, ensuring your writing is aligned to the academic journal standards. 

By following these steps, Paperpal not only simplifies the process of writing a research paper conclusion but also ensures it is impactful, concise, and aligned with academic standards. Sign up with Paperpal today and write your research paper conclusion 2x faster .  

The research paper conclusion is a crucial part of your paper as it provides the final opportunity to leave a strong impression on your readers. In the research paper conclusion, summarize the main points of your research paper by restating your research statement, highlighting the most important findings, addressing the research questions or objectives, explaining the broader context of the study, discussing the significance of your findings, providing recommendations if applicable, and emphasizing the takeaway message. The main purpose of the conclusion is to remind the reader of the main point or argument of your paper and to provide a clear and concise summary of the key findings and their implications. All these elements should feature on your list of what to put in the conclusion of a research paper to create a strong final statement for your work.

A strong conclusion is a critical component of a research paper, as it provides an opportunity to wrap up your arguments, reiterate your main points, and leave a lasting impression on your readers. Here are the key elements of a strong research paper conclusion: 1. Conciseness : A research paper conclusion should be concise and to the point. It should not introduce new information or ideas that were not discussed in the body of the paper. 2. Summarization : The research paper conclusion should be comprehensive enough to give the reader a clear understanding of the research’s main contributions. 3 . Relevance : Ensure that the information included in the research paper conclusion is directly relevant to the research paper’s main topic and objectives; avoid unnecessary details. 4 . Connection to the Introduction : A well-structured research paper conclusion often revisits the key points made in the introduction and shows how the research has addressed the initial questions or objectives. 5. Emphasis : Highlight the significance and implications of your research. Why is your study important? What are the broader implications or applications of your findings? 6 . Call to Action : Include a call to action or a recommendation for future research or action based on your findings.

The length of a research paper conclusion can vary depending on several factors, including the overall length of the paper, the complexity of the research, and the specific journal requirements. While there is no strict rule for the length of a conclusion, but it’s generally advisable to keep it relatively short. A typical research paper conclusion might be around 5-10% of the paper’s total length. For example, if your paper is 10 pages long, the conclusion might be roughly half a page to one page in length.

In general, you do not need to include citations in the research paper conclusion. Citations are typically reserved for the body of the paper to support your arguments and provide evidence for your claims. However, there may be some exceptions to this rule: 1. If you are drawing a direct quote or paraphrasing a specific source in your research paper conclusion, you should include a citation to give proper credit to the original author. 2. If your conclusion refers to or discusses specific research, data, or sources that are crucial to the overall argument, citations can be included to reinforce your conclusion’s validity.

The conclusion of a research paper serves several important purposes: 1. Summarize the Key Points 2. Reinforce the Main Argument 3. Provide Closure 4. Offer Insights or Implications 5. Engage the Reader. 6. Reflect on Limitations

Remember that the primary purpose of the research paper conclusion is to leave a lasting impression on the reader, reinforcing the key points and providing closure to your research. It’s often the last part of the paper that the reader will see, so it should be strong and well-crafted.

  • Makar, G., Foltz, C., Lendner, M., & Vaccaro, A. R. (2018). How to write effective discussion and conclusion sections. Clinical spine surgery, 31(8), 345-346.
  • Bunton, D. (2005). The structure of PhD conclusion chapters.  Journal of English for academic purposes ,  4 (3), 207-224.

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Home » Research Paper Conclusion – Writing Guide and Examples

Research Paper Conclusion – Writing Guide and Examples

Table of Contents

Research Paper Conclusion

Research Paper Conclusion

Definition:

A research paper conclusion is the final section of a research paper that summarizes the key findings, significance, and implications of the research. It is the writer’s opportunity to synthesize the information presented in the paper, draw conclusions, and make recommendations for future research or actions.

The conclusion should provide a clear and concise summary of the research paper, reiterating the research question or problem, the main results, and the significance of the findings. It should also discuss the limitations of the study and suggest areas for further research.

Parts of Research Paper Conclusion

The parts of a research paper conclusion typically include:

Restatement of the Thesis

The conclusion should begin by restating the thesis statement from the introduction in a different way. This helps to remind the reader of the main argument or purpose of the research.

Summary of Key Findings

The conclusion should summarize the main findings of the research, highlighting the most important results and conclusions. This section should be brief and to the point.

Implications and Significance

In this section, the researcher should explain the implications and significance of the research findings. This may include discussing the potential impact on the field or industry, highlighting new insights or knowledge gained, or pointing out areas for future research.

Limitations and Recommendations

It is important to acknowledge any limitations or weaknesses of the research and to make recommendations for how these could be addressed in future studies. This shows that the researcher is aware of the potential limitations of their work and is committed to improving the quality of research in their field.

Concluding Statement

The conclusion should end with a strong concluding statement that leaves a lasting impression on the reader. This could be a call to action, a recommendation for further research, or a final thought on the topic.

How to Write Research Paper Conclusion

Here are some steps you can follow to write an effective research paper conclusion:

  • Restate the research problem or question: Begin by restating the research problem or question that you aimed to answer in your research. This will remind the reader of the purpose of your study.
  • Summarize the main points: Summarize the key findings and results of your research. This can be done by highlighting the most important aspects of your research and the evidence that supports them.
  • Discuss the implications: Discuss the implications of your findings for the research area and any potential applications of your research. You should also mention any limitations of your research that may affect the interpretation of your findings.
  • Provide a conclusion : Provide a concise conclusion that summarizes the main points of your paper and emphasizes the significance of your research. This should be a strong and clear statement that leaves a lasting impression on the reader.
  • Offer suggestions for future research: Lastly, offer suggestions for future research that could build on your findings and contribute to further advancements in the field.

Remember that the conclusion should be brief and to the point, while still effectively summarizing the key findings and implications of your research.

Example of Research Paper Conclusion

Here’s an example of a research paper conclusion:

Conclusion :

In conclusion, our study aimed to investigate the relationship between social media use and mental health among college students. Our findings suggest that there is a significant association between social media use and increased levels of anxiety and depression among college students. This highlights the need for increased awareness and education about the potential negative effects of social media use on mental health, particularly among college students.

Despite the limitations of our study, such as the small sample size and self-reported data, our findings have important implications for future research and practice. Future studies should aim to replicate our findings in larger, more diverse samples, and investigate the potential mechanisms underlying the association between social media use and mental health. In addition, interventions should be developed to promote healthy social media use among college students, such as mindfulness-based approaches and social media detox programs.

Overall, our study contributes to the growing body of research on the impact of social media on mental health, and highlights the importance of addressing this issue in the context of higher education. By raising awareness and promoting healthy social media use among college students, we can help to reduce the negative impact of social media on mental health and improve the well-being of young adults.

Purpose of Research Paper Conclusion

The purpose of a research paper conclusion is to provide a summary and synthesis of the key findings, significance, and implications of the research presented in the paper. The conclusion serves as the final opportunity for the writer to convey their message and leave a lasting impression on the reader.

The conclusion should restate the research problem or question, summarize the main results of the research, and explain their significance. It should also acknowledge the limitations of the study and suggest areas for future research or action.

Overall, the purpose of the conclusion is to provide a sense of closure to the research paper and to emphasize the importance of the research and its potential impact. It should leave the reader with a clear understanding of the main findings and why they matter. The conclusion serves as the writer’s opportunity to showcase their contribution to the field and to inspire further research and action.

When to Write Research Paper Conclusion

The conclusion of a research paper should be written after the body of the paper has been completed. It should not be written until the writer has thoroughly analyzed and interpreted their findings and has written a complete and cohesive discussion of the research.

Before writing the conclusion, the writer should review their research paper and consider the key points that they want to convey to the reader. They should also review the research question, hypotheses, and methodology to ensure that they have addressed all of the necessary components of the research.

Once the writer has a clear understanding of the main findings and their significance, they can begin writing the conclusion. The conclusion should be written in a clear and concise manner, and should reiterate the main points of the research while also providing insights and recommendations for future research or action.

Characteristics of Research Paper Conclusion

The characteristics of a research paper conclusion include:

  • Clear and concise: The conclusion should be written in a clear and concise manner, summarizing the key findings and their significance.
  • Comprehensive: The conclusion should address all of the main points of the research paper, including the research question or problem, the methodology, the main results, and their implications.
  • Future-oriented : The conclusion should provide insights and recommendations for future research or action, based on the findings of the research.
  • Impressive : The conclusion should leave a lasting impression on the reader, emphasizing the importance of the research and its potential impact.
  • Objective : The conclusion should be based on the evidence presented in the research paper, and should avoid personal biases or opinions.
  • Unique : The conclusion should be unique to the research paper and should not simply repeat information from the introduction or body of the paper.

Advantages of Research Paper Conclusion

The advantages of a research paper conclusion include:

  • Summarizing the key findings : The conclusion provides a summary of the main findings of the research, making it easier for the reader to understand the key points of the study.
  • Emphasizing the significance of the research: The conclusion emphasizes the importance of the research and its potential impact, making it more likely that readers will take the research seriously and consider its implications.
  • Providing recommendations for future research or action : The conclusion suggests practical recommendations for future research or action, based on the findings of the study.
  • Providing closure to the research paper : The conclusion provides a sense of closure to the research paper, tying together the different sections of the paper and leaving a lasting impression on the reader.
  • Demonstrating the writer’s contribution to the field : The conclusion provides the writer with an opportunity to showcase their contribution to the field and to inspire further research and action.

Limitations of Research Paper Conclusion

While the conclusion of a research paper has many advantages, it also has some limitations that should be considered, including:

  • I nability to address all aspects of the research: Due to the limited space available in the conclusion, it may not be possible to address all aspects of the research in detail.
  • Subjectivity : While the conclusion should be objective, it may be influenced by the writer’s personal biases or opinions.
  • Lack of new information: The conclusion should not introduce new information that has not been discussed in the body of the research paper.
  • Lack of generalizability: The conclusions drawn from the research may not be applicable to other contexts or populations, limiting the generalizability of the study.
  • Misinterpretation by the reader: The reader may misinterpret the conclusions drawn from the research, leading to a misunderstanding of the findings.

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sample conclusion on research

How to Write Conclusion in Research Paper (With Example)

Writing a strong conclusion is a crucial part of any research paper. It provides a final opportunity to summarize your key findings, restate your thesis, and leave a lasting impression on your reader. However, many students struggle with how to effectively write a conclusion that ties everything together.

In this article, we’ll provide some tips and strategies for writing a compelling conclusion, along with an example to help illustrate the process. By following these guidelines, you can ensure that your research paper ends on a high note and leaves a lasting impact on your audience.

Why Conclusion is Important in Research Paper

The conclusion is the final chapter of your research paper journey, sealing the deal on all your hard work. After thoroughly laying out your main points and arguments in the body paragraphs, the conclusion gives you a chance to tie everything together into a neat, cohesive package.

More than just summarizing your key ideas, an effective conclusion shows readers the bigger picture of your research and why it matters. It highlights the significance of your findings , explains how your work contributes to the field, and points to potential future directions stemming from your study.

The conclusion is your last chance to leave a lasting impact and compel readers to seriously consider your perspective. With the right phrasing and tone, you can amplify the power of your work. Choose your words wisely, be persuasive yet diplomatic, and guide readers to walk away feeling satisfied by your reasoning and conclusions.

Approach the conclusion thoughtfully, reflect deeply on the larger meaning of your research, and craft impactful final sentences that linger in the reader’s mind. Wield your conclusion skillfully to make your research paper transformative and memorable. A powerful, thoughtful conclusion inspires action, sparks curiosity, and showcases the valuable insights you bring to the academic conversation.

How to Write Conclusion for a Research Paper

Crafting an effective conclusion in research paper requires thoughtful consideration and deliberate effort. After presenting your findings and analysis, the conclusion allows you to close your work with a flourish.

Begin by briefly summarizing the main points of your paper, provide a quick recap of your thesis, methodology, and key findings without repeating too much details from the body. Use this as an opportunity to reinforce your main argument and position within the field.

Next, highlight the significance and implications of your research. What new insights or perspectives does your work contribute? Discuss how your findings can inform future studies or practical applications. Convey why your research matters and how it moves the needle forward in your discipline.

Address any limitations of the current study and propose potential next steps that could be taken by you or other scholars to further the research. This shows readers you have critically considered ways to continue expanding knowledge in this area.

Finally, close with a memorable statement that captures the essence of your work and leaves a lasting impression. This could be an apt metaphor, a call to action, or a thought provoking question for readers to ponder. Choose words that will resonate with your audience and demonstrate the impact of your research.

With care and creativity, your conclusion can elevate your paper and cement your scholarly authority. Revisit often as you write to ensure your conclusion accomplishes its purpose, to convince readers of the value of your study and ignite further progress in your field.

What Not to Include in a Research Paper Conclusion

1. New Data: In a research paper conclusion, avoid presenting new data or evidence that wasn’t discussed earlier in the paper. It’s the time to summarize, analyze, or explain the significance of data already provided, not to introduce new material.

2. Irrelevant Details: The conclusion is not the spot for extraneous details not directly related to your research or its findings. Be focused and concise, tying up the paper neatly without going off-target.

3. Personal Opinions: Try not to include personal beliefs or subjective opinions unless your paper calls for it. Stick to empirical evidence, facts, and objective interpretation of your research.

4. Vague Summarization: While summarizing is the essence of a conclusion, too much of a broad or vague narrative should be avoided. Your conclusion shouldn’t be a generalization of the research but should specifically state your significant findings and their implications.

5. Overstating Results: No matter how exhilarating your research may be, don’t exaggerate its implications or general applications. Remember to acknowledge limitations or potential areas for future exploration.

6. Procrastinating: Refrain from leaving unresolved issues for future research. The conclusion is meant to tie up loose ends, not create more.

7. Repetition: While some reiteration is necessary, completely repeating the same phrases and points made previously can make your conclusion sound boring and redundant. Instead, try to look at your argument from a fresh, summarized perspective.

8. Apologies: Do not apologize or discredit your research efforts. Avoid phrases like, “This research was only” or “Although the study wasn’t able to prove”. A conclusion should confidently present your research results even if they’re unexpected or differ from your hypothesis.

9. Impractical Recommendations: While it’s often good to suggest directions for future research, don’t go overboard by proposing impractical or unachievable goals. Keep your recommendations relevant to your findings and within the realm of possibility.

10. Too Much Jargon: While it’s appropriate to use technical language throughout your research paper, remember the conclusion might be what a layman reads. Stick with a happy medium of professional lingo intermixed with understandable, plain language.

Also Check:   Conclusion for Internship Report

Conclusion in research Example

Research: Impact of Social Media Use on Adolescent Mental Health.

In conclusion, this study has demonstrated the significant impact of social media use on adolescent mental health. Our findings indicate that frequent social media use is associated with higher levels of anxiety and depression, particularly among girls. These results underscore the need for continued research in this area, as well as the development of interventions and strategies to promote healthy social media use among young people. By addressing this issue, we can help to ensure the well-being and success of the next generation.

Conclusion in research

Conclusion in Research Paper Example

Research: Impact of climate change on coral reefs in Florida.

In conclusion, the effect of climate change on Florida’s coral reefs presents a significant concern for the state’s ecosystem and economy. The data collected during this investigation reveal a direct correlation between rising ocean temperatures and coral bleaching events. This pattern has increased over the past decade, indicating that coral reefs’ health directly correlates with climate change effects.

Example Conclusion in Research

Research: The Influence of Social Media on Consumer Buying Behavior

Social media significantly shapes consumer buying behavior. Its power to influence is seen through peer opinions, online advertising, and brand communication. However, with the potential for misinformation, the reliability and quality of information are areas for further study. Despite these concerns, businesses leveraging social media can effectively boost their market reach and sales.

Conclusion in Research Paper Example

Research Paper Conclusion

Research: Impacts of Remote Work on Employee Productivity

Remote work has been found to notably enhance employee productivity. The elimination of commuting time, flexible scheduling, and comforting environment contribute to this increase. However, factors like home distractions and technological difficulties offer room for further research. Yet, integrating remote work can be a strategic pathway towards improved efficiency and workforce satisfaction.

These examples demonstrate techniques for crafting an effective conclusion in a research paper, providing your thesis with a powerful final statement. Now it is your turn to compose a strong concluding paragraph that summarizes your findings, reinforces your central argument, and leaves readers with a memorable takeaway.

Remember to restate your thesis without repeating it verbatim, highlight your main points without introducing new evidence, and end on a note that conveys the significance of your research. With a clear structure and purpose, proper grammar, and impactful writing, you can give your paper the persuasive conclusion it deserves.

Writing an effective conclusion takes practice, but by honing these skills you will elevate your academic writing to new heights. Use the strategies outlined here as a guide, believe in your capabilities, and soon you will be adept at concluding research papers powerfully. The final paragraph is your last chance to impress readers, so make it count!

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How to write a strong conclusion for your research paper

Last updated

17 February 2024

Reviewed by

Writing a research paper is a chance to share your knowledge and hypothesis. It's an opportunity to demonstrate your many hours of research and prove your ability to write convincingly.

Ideally, by the end of your research paper, you'll have brought your readers on a journey to reach the conclusions you've pre-determined. However, if you don't stick the landing with a good conclusion, you'll risk losing your reader’s trust.

Writing a strong conclusion for your research paper involves a few important steps, including restating the thesis and summing up everything properly.

Find out what to include and what to avoid, so you can effectively demonstrate your understanding of the topic and prove your expertise.

  • Why is a good conclusion important?

A good conclusion can cement your paper in the reader’s mind. Making a strong impression in your introduction can draw your readers in, but it's the conclusion that will inspire them.

  • What to include in a research paper conclusion

There are a few specifics you should include in your research paper conclusion. Offer your readers some sense of urgency or consequence by pointing out why they should care about the topic you have covered. Discuss any common problems associated with your topic and provide suggestions as to how these problems can be solved or addressed.

The conclusion should include a restatement of your initial thesis. Thesis statements are strengthened after you’ve presented supporting evidence (as you will have done in the paper), so make a point to reintroduce it at the end.

Finally, recap the main points of your research paper, highlighting the key takeaways you want readers to remember. If you've made multiple points throughout the paper, refer to the ones with the strongest supporting evidence.

  • Steps for writing a research paper conclusion

Many writers find the conclusion the most challenging part of any research project . By following these three steps, you'll be prepared to write a conclusion that is effective and concise.

  • Step 1: Restate the problem

Always begin by restating the research problem in the conclusion of a research paper. This serves to remind the reader of your hypothesis and refresh them on the main point of the paper. 

When restating the problem, take care to avoid using exactly the same words you employed earlier in the paper.

  • Step 2: Sum up the paper

After you've restated the problem, sum up the paper by revealing your overall findings. The method for this differs slightly, depending on whether you're crafting an argumentative paper or an empirical paper.

Argumentative paper: Restate your thesis and arguments

Argumentative papers involve introducing a thesis statement early on. In crafting the conclusion for an argumentative paper, always restate the thesis, outlining the way you've developed it throughout the entire paper.

It might be appropriate to mention any counterarguments in the conclusion, so you can demonstrate how your thesis is correct or how the data best supports your main points.

Empirical paper: Summarize research findings

Empirical papers break down a series of research questions. In your conclusion, discuss the findings your research revealed, including any information that surprised you.

Be clear about the conclusions you reached, and explain whether or not you expected to arrive at these particular ones.

  • Step 3: Discuss the implications of your research

Argumentative papers and empirical papers also differ in this part of a research paper conclusion. Here are some tips on crafting conclusions for argumentative and empirical papers.

Argumentative paper: Powerful closing statement

In an argumentative paper, you'll have spent a great deal of time expressing the opinions you formed after doing a significant amount of research. Make a strong closing statement in your argumentative paper's conclusion to share the significance of your work.

You can outline the next steps through a bold call to action, or restate how powerful your ideas turned out to be.

Empirical paper: Directions for future research

Empirical papers are broader in scope. They usually cover a variety of aspects and can include several points of view.

To write a good conclusion for an empirical paper, suggest the type of research that could be done in the future, including methods for further investigation or outlining ways other researchers might proceed.

If you feel your research had any limitations, even if they were outside your control, you could mention these in your conclusion.

After you finish outlining your conclusion, ask someone to read it and offer feedback. In any research project you're especially close to, it can be hard to identify problem areas. Having a close friend or someone whose opinion you value read the research paper and provide honest feedback can be invaluable. Take note of any suggested edits and consider incorporating them into your paper if they make sense.

  • Things to avoid in a research paper conclusion

Keep these aspects to avoid in mind as you're writing your conclusion and refer to them after you've created an outline.

Dry summary

Writing a memorable, succinct conclusion is arguably more important than a strong introduction. Take care to avoid just rephrasing your main points, and don't fall into the trap of repeating dry facts or citations.

You can provide a new perspective for your readers to think about or contextualize your research. Either way, make the conclusion vibrant and interesting, rather than a rote recitation of your research paper’s highlights.

Clichéd or generic phrasing

Your research paper conclusion should feel fresh and inspiring. Avoid generic phrases like "to sum up" or "in conclusion." These phrases tend to be overused, especially in an academic context and might turn your readers off.

The conclusion also isn't the time to introduce colloquial phrases or informal language. Retain a professional, confident tone consistent throughout your paper’s conclusion so it feels exciting and bold.

New data or evidence

While you should present strong data throughout your paper, the conclusion isn't the place to introduce new evidence. This is because readers are engaged in actively learning as they read through the body of your paper.

By the time they reach the conclusion, they will have formed an opinion one way or the other (hopefully in your favor!). Introducing new evidence in the conclusion will only serve to surprise or frustrate your reader.

Ignoring contradictory evidence

If your research reveals contradictory evidence, don't ignore it in the conclusion. This will damage your credibility as an expert and might even serve to highlight the contradictions.

Be as transparent as possible and admit to any shortcomings in your research, but don't dwell on them for too long.

Ambiguous or unclear resolutions

The point of a research paper conclusion is to provide closure and bring all your ideas together. You should wrap up any arguments you introduced in the paper and tie up any loose ends, while demonstrating why your research and data are strong.

Use direct language in your conclusion and avoid ambiguity. Even if some of the data and sources you cite are inconclusive or contradictory, note this in your conclusion to come across as confident and trustworthy.

  • Examples of research paper conclusions

Your research paper should provide a compelling close to the paper as a whole, highlighting your research and hard work. While the conclusion should represent your unique style, these examples offer a starting point:

Ultimately, the data we examined all point to the same conclusion: Encouraging a good work-life balance improves employee productivity and benefits the company overall. The research suggests that when employees feel their personal lives are valued and respected by their employers, they are more likely to be productive when at work. In addition, company turnover tends to be reduced when employees have a balance between their personal and professional lives. While additional research is required to establish ways companies can support employees in creating a stronger work-life balance, it's clear the need is there.

Social media is a primary method of communication among young people. As we've seen in the data presented, most young people in high school use a variety of social media applications at least every hour, including Instagram and Facebook. While social media is an avenue for connection with peers, research increasingly suggests that social media use correlates with body image issues. Young girls with lower self-esteem tend to use social media more often than those who don't log onto social media apps every day. As new applications continue to gain popularity, and as more high school students are given smartphones, more research will be required to measure the effects of prolonged social media use.

What are the different kinds of research paper conclusions?

There are no formal types of research paper conclusions. Ultimately, the conclusion depends on the outline of your paper and the type of research you’re presenting. While some experts note that research papers can end with a new perspective or commentary, most papers should conclude with a combination of both. The most important aspect of a good research paper conclusion is that it accurately represents the body of the paper.

Can I present new arguments in my research paper conclusion?

Research paper conclusions are not the place to introduce new data or arguments. The body of your paper is where you should share research and insights, where the reader is actively absorbing the content. By the time a reader reaches the conclusion of the research paper, they should have formed their opinion. Introducing new arguments in the conclusion can take a reader by surprise, and not in a positive way. It might also serve to frustrate readers.

How long should a research paper conclusion be?

There's no set length for a research paper conclusion. However, it's a good idea not to run on too long, since conclusions are supposed to be succinct. A good rule of thumb is to keep your conclusion around 5 to 10 percent of the paper's total length. If your paper is 10 pages, try to keep your conclusion under one page.

What should I include in a research paper conclusion?

A good research paper conclusion should always include a sense of urgency, so the reader can see how and why the topic should matter to them. You can also note some recommended actions to help fix the problem and some obstacles they might encounter. A conclusion should also remind the reader of the thesis statement, along with the main points you covered in the paper. At the end of the conclusion, add a powerful closing statement that helps cement the paper in the mind of the reader.

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  • How to Write Discussions and Conclusions

How to Write Discussions and Conclusions

The discussion section contains the results and outcomes of a study. An effective discussion informs readers what can be learned from your experiment and provides context for the results.

What makes an effective discussion?

When you’re ready to write your discussion, you’ve already introduced the purpose of your study and provided an in-depth description of the methodology. The discussion informs readers about the larger implications of your study based on the results. Highlighting these implications while not overstating the findings can be challenging, especially when you’re submitting to a journal that selects articles based on novelty or potential impact. Regardless of what journal you are submitting to, the discussion section always serves the same purpose: concluding what your study results actually mean.

A successful discussion section puts your findings in context. It should include:

  • the results of your research,
  • a discussion of related research, and
  • a comparison between your results and initial hypothesis.

Tip: Not all journals share the same naming conventions.

You can apply the advice in this article to the conclusion, results or discussion sections of your manuscript.

Our Early Career Researcher community tells us that the conclusion is often considered the most difficult aspect of a manuscript to write. To help, this guide provides questions to ask yourself, a basic structure to model your discussion off of and examples from published manuscripts. 

sample conclusion on research

Questions to ask yourself:

  • Was my hypothesis correct?
  • If my hypothesis is partially correct or entirely different, what can be learned from the results? 
  • How do the conclusions reshape or add onto the existing knowledge in the field? What does previous research say about the topic? 
  • Why are the results important or relevant to your audience? Do they add further evidence to a scientific consensus or disprove prior studies? 
  • How can future research build on these observations? What are the key experiments that must be done? 
  • What is the “take-home” message you want your reader to leave with?

How to structure a discussion

Trying to fit a complete discussion into a single paragraph can add unnecessary stress to the writing process. If possible, you’ll want to give yourself two or three paragraphs to give the reader a comprehensive understanding of your study as a whole. Here’s one way to structure an effective discussion:

sample conclusion on research

Writing Tips

While the above sections can help you brainstorm and structure your discussion, there are many common mistakes that writers revert to when having difficulties with their paper. Writing a discussion can be a delicate balance between summarizing your results, providing proper context for your research and avoiding introducing new information. Remember that your paper should be both confident and honest about the results! 

What to do

  • Read the journal’s guidelines on the discussion and conclusion sections. If possible, learn about the guidelines before writing the discussion to ensure you’re writing to meet their expectations. 
  • Begin with a clear statement of the principal findings. This will reinforce the main take-away for the reader and set up the rest of the discussion. 
  • Explain why the outcomes of your study are important to the reader. Discuss the implications of your findings realistically based on previous literature, highlighting both the strengths and limitations of the research. 
  • State whether the results prove or disprove your hypothesis. If your hypothesis was disproved, what might be the reasons? 
  • Introduce new or expanded ways to think about the research question. Indicate what next steps can be taken to further pursue any unresolved questions. 
  • If dealing with a contemporary or ongoing problem, such as climate change, discuss possible consequences if the problem is avoided. 
  • Be concise. Adding unnecessary detail can distract from the main findings. 

What not to do

Don’t

  • Rewrite your abstract. Statements with “we investigated” or “we studied” generally do not belong in the discussion. 
  • Include new arguments or evidence not previously discussed. Necessary information and evidence should be introduced in the main body of the paper. 
  • Apologize. Even if your research contains significant limitations, don’t undermine your authority by including statements that doubt your methodology or execution. 
  • Shy away from speaking on limitations or negative results. Including limitations and negative results will give readers a complete understanding of the presented research. Potential limitations include sources of potential bias, threats to internal or external validity, barriers to implementing an intervention and other issues inherent to the study design. 
  • Overstate the importance of your findings. Making grand statements about how a study will fully resolve large questions can lead readers to doubt the success of the research. 

Snippets of Effective Discussions:

Consumer-based actions to reduce plastic pollution in rivers: A multi-criteria decision analysis approach

Identifying reliable indicators of fitness in polar bears

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How to Write a Conclusion for a Research Paper

How to Write a Conclusion for a Research Paper

3-minute read

  • 29th August 2023

If you’re writing a research paper, the conclusion is your opportunity to summarize your findings and leave a lasting impression on your readers. In this post, we’ll take you through how to write an effective conclusion for a research paper and how you can:

·   Reword your thesis statement

·   Highlight the significance of your research

·   Discuss limitations

·   Connect to the introduction

·   End with a thought-provoking statement

Rewording Your Thesis Statement

Begin your conclusion by restating your thesis statement in a way that is slightly different from the wording used in the introduction. Avoid presenting new information or evidence in your conclusion. Just summarize the main points and arguments of your essay and keep this part as concise as possible. Remember that you’ve already covered the in-depth analyses and investigations in the main body paragraphs of your essay, so it’s not necessary to restate these details in the conclusion.

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Highlighting the Significance of Your Research

The conclusion is a good place to emphasize the implications of your research . Avoid ambiguous or vague language such as “I think” or “maybe,” which could weaken your position. Clearly explain why your research is significant and how it contributes to the broader field of study.

Here’s an example from a (fictional) study on the impact of social media on mental health:

Discussing Limitations

Although it’s important to emphasize the significance of your study, you can also use the conclusion to briefly address any limitations you discovered while conducting your research, such as time constraints or a shortage of resources. Doing this demonstrates a balanced and honest approach to your research.

Connecting to the Introduction

In your conclusion, you can circle back to your introduction , perhaps by referring to a quote or anecdote you discussed earlier. If you end your paper on a similar note to how you began it, you will create a sense of cohesion for the reader and remind them of the meaning and significance of your research.

Ending With a Thought-Provoking Statement

Consider ending your paper with a thought-provoking and memorable statement that relates to the impact of your research questions or hypothesis. This statement can be a call to action, a philosophical question, or a prediction for the future (positive or negative). Here’s an example that uses the same topic as above (social media and mental health):

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sample conclusion on research

How to Write a Conclusion for a Research Paper

sample conclusion on research

When you're wrapping up a research paper, the conclusion is like the grand finale of a fireworks show – it's your chance to leave a lasting impression. In this article, we'll break down the steps to help you write a winning research paper conclusion that not only recaps your main points but also ties everything together. Consider it the "So what?" moment – why should people care about your research? Our professional essay writers will guide you through making your conclusion strong, clear, and something that sticks with your readers long after they've put down your paper. So, let's dive in and ensure your research ends on a high note!

What Is a Conclusion in a Research Paper

In a research paper, the conclusion serves as the final segment, where you summarize the main points and findings of your study. It's not just a repetition of what you've already said but rather a chance to tie everything together and highlight the significance of your research. As you learn how to start a research paper , a good conclusion also often discusses the implications of your findings, suggests potential areas for further research, and leaves the reader with a lasting impression of the importance and relevance of your work in the broader context of the field. Essentially, it's your last opportunity to make a strong impact and leave your readers with a clear understanding of the significance of your research. Here’s a research paper conclusion example:

In conclusion, this research paper has navigated the intricacies of sustainable urban development, shedding light on the pivotal role of community engagement and innovative planning strategies. Through applying qualitative and quantitative research methods, we've uncovered valuable insights into the challenges and opportunities inherent in fostering environmentally friendly urban spaces. The implications of these findings extend beyond the confines of this study, emphasizing the imperative for continued exploration in the realms of urban planning and environmental sustainability. By emphasizing both the practical applications and theoretical contributions, this research underscores the significance of community involvement and forward-thinking strategies in shaping the future of urban landscapes. As cities evolve, incorporating these insights into planning and development practices will create resilient and harmonious urban environments.

Conclusion Outline for Research Paper

This outline for a research paper conclusion provides a structured framework to ensure that your ending effectively summarizes the key elements of your research paper and leaves a lasting impression on your readers. Adjust the content based on the specific requirements and focus of your research.

Restate the Thesis Statement

  • Briefly restate the main thesis or research question.
  • Emphasize the core objective or purpose of the study.

Summarize Key Findings

  • Recap the main points and key findings from each section of the paper.
  • Provide a concise overview of the research journey.

Discuss Implications

  • Explore the broader implications of the research findings.
  • Discuss how the results contribute to the existing body of knowledge in the field.

Address Limitations

  • Acknowledge any limitations or constraints encountered during the research process.
  • Explain how these limitations may impact the interpretation of the findings.

Suggest Areas for Future Research

  • Propose potential directions for future studies related to the topic.
  • Identify gaps in the current research that warrant further exploration.

Reaffirm Significance

  • Reaffirm the importance and relevance of the research in the broader context.
  • Highlight the practical applications or real-world implications of the study.

Concluding Statement

  • Craft a strong, memorable closing statement that leaves a lasting impression.
  • Sum up the overall impact of the research and its potential contribution to the field.

Study the full guide on how to make a research paper outline here, which will also specify the conclusion writing specifics to improve your general prowess.

Tips on How to Make a Conclusion in Research

Here are key considerations regarding a conclusion for research paper to not only recap the primary ideas in your work but also delve deeper to earn a higher grade:

Research Paper Conclusion

  • Provide a concise recap of your main research outcomes.
  • Remind readers of your research goals and their accomplishments.
  • Stick to summarizing existing content; refrain from adding new details.
  • Emphasize why your research matters and its broader implications.
  • Clearly explain the practical or theoretical impact of your findings.
  • Prompt readers to reflect on how your research influences their perspective.
  • Briefly discuss the robustness of your research methods.
  • End with a suggestion for future research or a practical application.
  • Transparently address any constraints or biases in your study.
  • End on a powerful note, leaving a memorable impression on your readers.

devices in research paper conclusion

For your inspiration, we’ve also prepared this research proposal example APA , which dwells on another important aspect of research writing.

How to Write a Research Paper Conclusion

As you finish your research paper, the conclusion takes center stage. In this section, we've got five practical tips for writing a conclusion for a research paper. We'll guide you through summarizing your key findings, revisiting your research goals, discussing the bigger picture, addressing any limitations, and ending on a powerful note. Think of it as your roadmap to creating a conclusion that not only wraps up your research but also leaves a lasting impact on your readers. Let's dive in and make sure your conclusion stands out for all the right reasons!

How to Write a Research Paper Conclusion

Synthesize Core Discoveries. Initiate your conclusion by synthesizing the essential discoveries of your research. Offer a succinct recapitulation of the primary points and outcomes you have elucidated in your paper. This aids in reinforcing the gravity of your work and reiterates the pivotal information you have presented.

Revisit Research Objectives. Revisit the research objectives or questions you outlined at the beginning of your paper. Assess whether you have successfully addressed these objectives and if your findings align with the initial goals of your research. This reflection helps tie your conclusion back to the purpose of your study.

Discuss Implications and Contributions. Discuss the broader implications of your research and its potential contributions to the field. Consider how your findings might impact future research, applications, or understanding of the subject matter. This demonstrates the significance of your work and places it within a larger context.

Address Limitations and Future Research. Acknowledge any limitations in your study, such as constraints in data collection or potential biases. Briefly discuss how these limitations might have affected your results. Additionally, suggest areas for future research that could build upon your work, addressing any unanswered questions or unexplored aspects. This demonstrates a thoughtful approach to your research.

End with a Strong Conclusion Statement. Conclude your research paper with a strong and memorable statement that reinforces the key message you want readers to take away. This could be a call to action, a proposal for further investigation, or a reflection on the broader significance of your findings. Leave your readers with a lasting impression that emphasizes the importance of your research. Remember that you can buy a research paper anytime if you lack time or get stuck in writer’s block.

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Stylistic Devices to Use in a Conclusion

Discover distinctive stylistic insights that you can apply when writing a conclusion for a research paper:

  • Rhetorical Questions. When using rhetorical questions, strategically place them to engage readers' minds. For instance, you might pose a question that prompts reflection on the broader implications of your findings, leaving your audience with something to ponder.
  • Powerful Language. Incorporate strong language to convey a sense of conviction and importance. Choose words that resonate with the overall tone of your research and amplify the significance of your conclusions. This adds weight to your key messages.
  • Repetitions. Repetitions can be employed to reinforce essential ideas. Reiterate key phrases or concepts in a way that emphasizes their importance without sounding redundant. This technique serves to drive home your main points.
  • Anecdotes. Integrating anecdotes into your conclusion can provide a human touch. Share a brief and relevant story that connects with your research, making the information more relatable and memorable for your audience.
  • Vivid Imagery. Lastly, use vivid imagery to paint a picture in the minds of your readers. Appeal to their senses by describing scenarios or outcomes related to your research. This creates a more immersive and lasting impression.

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How to Make a Conclusion Logically Appealing

Knowing how to write a conclusion for a research paper that is logically appealing is important for leaving a lasting impression on your readers. Here are some tips to achieve this:

Logical Sequencing

  • Present your conclusion in a structured manner, following the natural flow of your paper. Readers should effortlessly follow your thought process, making your conclusion more accessible and persuasive.

Reinforce Main Arguments

  • Emphasize the core arguments and findings from your research. By reinforcing key points, you solidify your stance and provide a logical culmination to your paper.

Address Counterarguments

  • Acknowledge and address potential counterarguments or limitations in your research. Demonstrate intellectual honesty and strengthen your conclusion by preemptively addressing potential doubts.

Connect with Introduction

  • Revisit themes or concepts introduced in your introduction to create a cohesive narrative, allowing readers to trace the logical progression of your research from start to finish.

Propose Actionable Insights

  • Suggest practical applications or recommendations based on your findings. This will add a forward-looking dimension, making your conclusion more relevant and compelling.

Highlight Significance

  • Clearly articulate the broader implications of your research to convey the importance of your work and its potential impact on the field, making your conclusion logically compelling.

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Avoid These Things When Writing a Research Paper Conclusion

As you write your conclusion of research paper, there’s a list of things professional writers don’t recommend doing. Consider these issues carefully:

Avoid in Your Research Paper Conclusion

  • Repetition of Exact Phrases
  • Repetitively using the same phrases or sentences from the main body. Repetition can make your conclusion seem redundant and less engaging.
  • Overly Lengthy Summaries
  • Providing excessively detailed summaries of each section of your paper. Readers may lose interest if the conclusion becomes too long and detailed.
  • Unclear Connection to the Introduction
  • Failing to connect the conclusion back to the introduction. A lack of continuity may make the paper feel disjointed.
  • Adding New Arguments or Ideas
  • Introducing new arguments or ideas that were not addressed in the body. This can confuse the reader and disrupt the coherence of your paper.
  • Overuse of Complex Jargon
  • Using excessively complex or technical language without clarification. Clear communication is essential in the conclusion, ensuring broad understanding.
  • Apologizing or Undermining Confidence
  • Apologizing for limitations or expressing doubt about your work. Maintain a confident tone; if limitations exist, present them objectively without undermining your research.
  • Sweeping Generalizations
  • Making overly broad or unsupported generalizations. Such statements can weaken the credibility of your conclusion.
  • Neglecting the Significance
  • Failing to emphasize the broader significance of your research. Readers need to understand why your findings matter in a larger context.
  • Abrupt Endings
  • Concluding abruptly without a strong closing statement. A powerful ending leaves a lasting impression; avoid a sudden or weak conclusion.

Research Paper Conclusion Example

That covers the essential aspects of summarizing a research paper. The only remaining step is to review the conclusion examples for research paper provided by our team.

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Final Thoughts

In conclusion, the knowledge of how to write the conclusion of a research paper is pivotal for presenting your findings and leaving a lasting impression on your readers. By summarizing the key points, reiterating the significance of your research, and offering avenues for future exploration, you can create a conclusion that not only reinforces the value of your study but also encourages further academic discourse. Remember to balance brevity and completeness, ensuring your conclusion is concise yet comprehensive. Emphasizing the practical implications of your research and connecting it to the broader academic landscape will help solidify the impact of your work. Pay someone to write a research paper if you are having a hard time finishing your coursework on time.

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How to Write a Conclusion for a Research Paper

Last Updated: June 29, 2023 Approved

This article was co-authored by Christopher Taylor, PhD . Christopher Taylor is an Adjunct Assistant Professor of English at Austin Community College in Texas. He received his PhD in English Literature and Medieval Studies from the University of Texas at Austin in 2014. wikiHow marks an article as reader-approved once it receives enough positive feedback. This article received 42 testimonials and 82% of readers who voted found it helpful, earning it our reader-approved status. This article has been viewed 2,255,952 times.

The conclusion of a research paper needs to summarize the content and purpose of the paper without seeming too wooden or dry. Every basic conclusion must share several key elements, but there are also several tactics you can play around with to craft a more effective conclusion and several you should avoid to prevent yourself from weakening your paper's conclusion. Here are some writing tips to keep in mind when creating a conclusion for your next research paper.

Sample Conclusions

Writing a basic conclusion.

Step 1 Restate the topic.

  • Do not spend a great amount of time or space restating your topic.
  • A good research paper will make the importance of your topic apparent, so you do not need to write an elaborate defense of your topic in the conclusion.
  • Usually a single sentence is all you need to restate your topic.
  • An example would be if you were writing a paper on the epidemiology of infectious disease, you might say something like "Tuberculosis is a widespread infectious disease that affects millions of people worldwide every year."
  • Yet another example from the humanities would be a paper about the Italian Renaissance: "The Italian Renaissance was an explosion of art and ideas centered around artists, writers, and thinkers in Florence."

Step 2 Restate your thesis.

  • A thesis is a narrowed, focused view on the topic at hand.
  • This statement should be rephrased from the thesis you included in your introduction. It should not be identical or too similar to the sentence you originally used.
  • Try re-wording your thesis statement in a way that complements your summary of the topic of your paper in your first sentence of your conclusion.
  • An example of a good thesis statement, going back to the paper on tuberculosis, would be "Tuberculosis is a widespread disease that affects millions of people worldwide every year. Due to the alarming rate of the spread of tuberculosis, particularly in poor countries, medical professionals are implementing new strategies for the diagnosis, treatment, and containment of this disease ."

Step 3 Briefly summarize your main points.

  • A good way to go about this is to re-read the topic sentence of each major paragraph or section in the body of your paper.
  • Find a way to briefly restate each point mentioned in each topic sentence in your conclusion. Do not repeat any of the supporting details used within your body paragraphs.
  • Under most circumstances, you should avoid writing new information in your conclusion. This is especially true if the information is vital to the argument or research presented in your paper.
  • For example, in the TB paper you could summarize the information. "Tuberculosis is a widespread disease that affects millions of people worldwide. Due to the alarming rate of the spread of tuberculosis, particularly in poor countries, medical professionals are implementing new strategies for the diagnosis, treatment, and containment of this disease. In developing countries, such as those in Africa and Southeast Asia, the rate of TB infections is soaring. Crowded conditions, poor sanitation, and lack of access to medical care are all compounding factors in the spread of the disease. Medical experts, such as those from the World Health Organization are now starting campaigns to go into communities in developing countries and provide diagnostic testing and treatments. However, the treatments for TB are very harsh and have many side effects. This leads to patient non-compliance and spread of multi-drug resistant strains of the disease."

Step 4 Add the points up.

  • Note that this is not needed for all research papers.
  • If you already fully explained what the points in your paper mean or why they are significant, you do not need to go into them in much detail in your conclusion. Simply restating your thesis or the significance of your topic should suffice.
  • It is always best practice to address important issues and fully explain your points in the body of your paper. The point of a conclusion to a research paper is to summarize your argument for the reader and, perhaps, to call the reader to action if needed.

Step 5 Make a call to action when appropriate.

  • Note that a call for action is not essential to all conclusions. A research paper on literary criticism, for instance, is less likely to need a call for action than a paper on the effect that television has on toddlers and young children.
  • A paper that is more likely to call readers to action is one that addresses a public or scientific need. Let's go back to our example of tuberculosis. This is a very serious disease that is spreading quickly and with antibiotic-resistant forms.
  • A call to action in this research paper would be a follow-up statement that might be along the lines of "Despite new efforts to diagnose and contain the disease, more research is needed to develop new antibiotics that will treat the most resistant strains of tuberculosis and ease the side effects of current treatments."

Step 6 Answer the “so what” question.

  • For example, if you are writing a history paper, then you might discuss how the historical topic you discussed matters today. If you are writing about a foreign country, then you might use the conclusion to discuss how the information you shared may help readers understand their own country.

Making Your Conclusion as Effective as Possible

Step 1 Stick with a basic synthesis of information.

  • Since this sort of conclusion is so basic, you must aim to synthesize the information rather than merely summarizing it.
  • Instead of merely repeating things you already said, rephrase your thesis and supporting points in a way that ties them all together.
  • By doing so, you make your research paper seem like a "complete thought" rather than a collection of random and vaguely related ideas.

Step 2 Bring things full circle.

  • Ask a question in your introduction. In your conclusion, restate the question and provide a direct answer.
  • Write an anecdote or story in your introduction but do not share the ending. Instead, write the conclusion to the anecdote in the conclusion of your paper.
  • For example, if you wanted to get more creative and put a more humanistic spin on a paper on tuberculosis, you might start your introduction with a story about a person with the disease, and refer to that story in your conclusion. For example, you could say something like this before you re-state your thesis in your conclusion: "Patient X was unable to complete the treatment for tuberculosis due to severe side effects and unfortunately succumbed to the disease."
  • Use the same concepts and images introduced in your introduction in your conclusion. The images may or may not appear at other points throughout the research paper.

Step 3 Close with logic.

  • Include enough information about your topic to back the statement up but do not get too carried away with excess detail.
  • If your research did not provide you with a clear-cut answer to a question posed in your thesis, do not be afraid to indicate as much.
  • Restate your initial hypothesis and indicate whether you still believe it or if the research you performed has begun swaying your opinion.
  • Indicate that an answer may still exist and that further research could shed more light on the topic at hand.

Step 4 Pose a question.

  • This may not be appropriate for all types of research papers. Most research papers, such as one on effective treatment for diseases, will have the information to make the case for a particular argument already in the paper.
  • A good example of a paper that might ask a question of the reader in the ending is one about a social issue, such as poverty or government policy.
  • Ask a question that will directly get at the heart or purpose of the paper. This question is often the same question, or some version of it, that you may have started with when you began your research.
  • Make sure that the question can be answered by the evidence presented in your paper.
  • If desired you can briefly summarize the answer after stating the question. You could also leave the question hanging for the reader to answer, though.

Step 5 Make a suggestion.

  • Even without a call to action, you can still make a recommendation to your reader.
  • For instance, if you are writing about a topic like third-world poverty, you can various ways for the reader to assist in the problem without necessarily calling for more research.
  • Another example would be, in a paper about treatment for drug-resistant tuberculosis, you could suggest donating to the World Health Organization or research foundations that are developing new treatments for the disease.

Avoiding Common Pitfalls

Step 1 Avoid saying

  • These sayings usually sound stiff, unnatural, or trite when used in writing.
  • Moreover, using a phrase like "in conclusion" to begin your conclusion is a little too straightforward and tends to lead to a weak conclusion. A strong conclusion can stand on its own without being labeled as such.

Step 2 Do not wait until the conclusion to state your thesis.

  • Always state the main argument or thesis in the introduction. A research paper is an analytical discussion of an academic topic, not a mystery novel.
  • A good, effective research paper will allow your reader to follow your main argument from start to finish.
  • This is why it is best practice to start your paper with an introduction that states your main argument and to end the paper with a conclusion that re-states your thesis for re-iteration.

Step 3 Leave out new information.

  • All significant information should be introduced in the body of the paper.
  • Supporting evidence expands the topic of your paper by making it appear more detailed. A conclusion should narrow the topic to a more general point.
  • A conclusion should only summarize what you have already stated in the body of your paper.
  • You may suggest further research or a call to action, but you should not bring in any new evidence or facts in the conclusion.

Step 4 Avoid changing the tone of the paper.

  • Most often, a shift in tone occurs when a research paper with an academic tone gives an emotional or sentimental conclusion.
  • Even if the topic of the paper is of personal significance for you, you should not indicate as much in your paper.
  • If you want to give your paper a more humanistic slant, you could start and end your paper with a story or anecdote that would give your topic more personal meaning to the reader.
  • This tone should be consistent throughout the paper, however.

Step 5 Make no apologies.

  • Apologetic statements include phrases like "I may not be an expert" or "This is only my opinion."
  • Statements like this can usually be avoided by refraining from writing in the first-person.
  • Avoid any statements in the first-person. First-person is generally considered to be informal and does not fit with the formal tone of a research paper.

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  • ↑ http://owl.english.purdue.edu/owl/resource/724/04/
  • ↑ http://www.crlsresearchguide.org/18_Writing_Conclusion.asp
  • ↑ http://writing.wisc.edu/Handbook/PlanResearchPaper.html#conclusion
  • ↑ http://writingcenter.unc.edu/handouts/conclusions/
  • ↑ http://writing2.richmond.edu/writing/wweb/conclude.html

About This Article

Christopher Taylor, PhD

To write a conclusion for a research paper, start by restating your thesis statement to remind your readers what your main topic is and bring everything full circle. Then, briefly summarize all of the main points you made throughout your paper, which will help remind your readers of everything they learned. You might also want to include a call to action if you think more research or work needs to be done on your topic by writing something like, "Despite efforts to contain the disease, more research is needed to develop antibiotics." Finally, end your conclusion by explaining the broader context of your topic and why your readers should care about it, which will help them understand why your topic is relevant and important. For tips from our Academic co-author, like how to avoid common pitfalls when writing your conclusion, scroll down! Did this summary help you? Yes No

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How to Write a Research Paper Conclusion Section

sample conclusion on research

What is a conclusion in a research paper?

The conclusion in a research paper is the final paragraph or two in a research paper. In scientific papers, the conclusion usually follows the Discussion section , summarizing the importance of the findings and reminding the reader why the work presented in the paper is relevant.

However, it can be a bit confusing to distinguish the conclusion section/paragraph from a summary or a repetition of your findings, your own opinion, or the statement of the implications of your work. In fact, the conclusion should contain a bit of all of these other parts but go beyond it—but not too far beyond! 

The structure and content of the conclusion section can also vary depending on whether you are writing a research manuscript or an essay. This article will explain how to write a good conclusion section, what exactly it should (and should not) contain, how it should be structured, and what you should avoid when writing it.  

Table of Contents:

What does a good conclusion section do, what to include in a research paper conclusion.

  • Conclusion in an Essay
  • Research Paper Conclusion 
  • Conclusion Paragraph Outline and Example
  • What Not to Do When Writing a Conclusion

The conclusion of a research paper has several key objectives. It should:

  • Restate your research problem addressed in the introduction section
  • Summarize your main arguments, important findings, and broader implications
  • Synthesize key takeaways from your study

The specific content in the conclusion depends on whether your paper presents the results of original scientific research or constructs an argument through engagement with previously published sources.

You presented your general field of study to the reader in the introduction section, by moving from general information (the background of your work, often combined with a literature review ) to the rationale of your study and then to the specific problem or topic you addressed, formulated in the form of the statement of the problem in research or the thesis statement in an essay.

In the conclusion section, in contrast, your task is to move from your specific findings or arguments back to a more general depiction of how your research contributes to the readers’ understanding of a certain concept or helps solve a practical problem, or fills an important gap in the literature. The content of your conclusion section depends on the type of research you are doing and what type of paper you are writing. But whatever the outcome of your work is, the conclusion is where you briefly summarize it and place it within a larger context. It could be called the “take-home message” of the entire paper.

What to summarize in the conclusion

Your conclusion section needs to contain a very brief summary of your work , a very brief summary of the main findings of your work, and a mention of anything else that seems relevant when you now look at your work from a bigger perspective, even if it was not initially listed as one of your main research questions. This could be a limitation, for example, a problem with the design of your experiment that either needs to be considered when drawing any conclusions or that led you to ask a different question and therefore draw different conclusions at the end of your study (compared to when you started out).

Once you have reminded the reader of what you did and what you found, you need to go beyond that and also provide either your own opinion on why your work is relevant (and for whom, and how) or theoretical or practical implications of the study , or make a specific call for action if there is one to be made.   

How to Write an Essay Conclusion

Academic essays follow quite different structures than their counterparts in STEM and the natural sciences. Humanities papers often have conclusion sections that are much longer and contain more detail than scientific papers. There are three main types of academic essay conclusions.

Summarizing conclusion

The most typical conclusion at the end of an analytical/explanatory/argumentative essay is a summarizing conclusion . This is, as the name suggests, a clear summary of the main points of your topic and thesis. Since you might have gone through a number of different arguments or subtopics in the main part of your essay, you need to remind the reader again what those were, how they fit into each other, and how they helped you develop or corroborate your hypothesis.

For an essay that analyzes how recruiters can hire the best candidates in the shortest time or on “how starving yourself will increase your lifespan, according to science”, a summary of all the points you discussed might be all you need. Note that you should not exactly repeat what you said earlier, but rather highlight the essential details and present those to your reader in a different way. 

Externalizing conclusion

If you think that just reminding the reader of your main points is not enough, you can opt for an externalizing conclusion instead, that presents new points that were not presented in the paper so far. These new points can be additional facts and information or they can be ideas that are relevant to the topic and have not been mentioned before.

Such a conclusion can stimulate your readers to think about your topic or the implications of your analysis in a whole new way. For example, at the end of a historical analysis of a specific event or development, you could direct your reader’s attention to some current events that were not the topic of your essay but that provide a different context for your findings.

Editorial conclusion

In an editorial conclusion , another common type of conclusion that you will find at the end of papers and essays, you do not add new information but instead present your own experiences or opinions on the topic to round everything up. What makes this type of conclusion interesting is that you can choose to agree or disagree with the information you presented in your paper so far. For example, if you have collected and analyzed information on how a specific diet helps people lose weight, you can nevertheless have your doubts on the sustainability of that diet or its practicability in real life—if such arguments were not included in your original thesis and have therefore not been covered in the main part of your paper, the conclusion section is the place where you can get your opinion across.    

How to Conclude an Empirical Research Paper

An empirical research paper is usually more concise and succinct than an essay, because, if it is written well, it focuses on one specific question, describes the method that was used to answer that one question, describes and explains the results, and guides the reader in a logical way from the introduction to the discussion without going on tangents or digging into not absolutely relevant topics.

Summarize the findings

In a scientific paper, you should include a summary of the findings. Don’t go into great detail here (you will have presented your in-depth  results  and  discussion  already), but do clearly express the answers to the  research questions  you investigated.

Describe your main findings, even if they weren’t necessarily the ones anticipated, and explain the conclusion they led you to. Explain these findings in as few words as possible.

Instead of beginning with “ In conclusion, in this study, we investigated the effect of stress on the brain using fMRI …”, you should try to find a way to incorporate the repetition of the essential (and only the essential) details into the summary of the key points. “ The findings of this fMRI study on the effect of stress on the brain suggest that …” or “ While it has been known for a long time that stress has an effect on the brain, the findings of this fMRI study show that, surprisingly… ” would be better ways to start a conclusion. 

You should also not bring up new ideas or present new facts in the conclusion of a research paper, but stick to the background information you have presented earlier, to the findings you have already discussed, and the limitations and implications you have already described. The one thing you can add here is a practical recommendation that you haven’t clearly stated before—but even that one needs to follow logically from everything you have already discussed in the discussion section.

Discuss the implications

After summing up your key arguments or findings, conclude the paper by stating the broader implications of the research , whether in methods , approach, or findings. Express practical or theoretical takeaways from your paper. This often looks like a “call to action” or a final “sales pitch” that puts an exclamation point on your paper.

If your research topic is more theoretical in nature, your closing statement should express the significance of your argument—for example, in proposing a new understanding of a topic or laying the groundwork for future research.

Future research example

Future research into education standards should focus on establishing a more detailed picture of how novel pedagogical approaches impact young people’s ability to absorb new and difficult concepts. Moreover, observational studies are needed to gain more insight into how specific teaching models affect the retention of relationships and facts—for instance, how inquiry-based learning and its emphasis on lateral thinking can be used as a jumping-off point for more holistic classroom approaches.

Research Conclusion Example and Outline

Let’s revisit the study on the effect of stress on the brain we mentioned before and see what the common structure for a conclusion paragraph looks like, in three steps. Following these simple steps will make it easy for you to wrap everything up in one short paragraph that contains all the essential information: 

One: Short summary of what you did, but integrated into the summary of your findings:

While it has been known for a long time that stress has an effect on the brain, the findings of this fMRI study in 25 university students going through mid-term exams show that, surprisingly, one’s attitude to the experienced stress significantly modulates the brain’s response to it. 

Note that you don’t need to repeat any methodological or technical details here—the reader has been presented with all of these before, they have read your results section and the discussion of your results, and even (hopefully!) a discussion of the limitations and strengths of your paper. The only thing you need to remind them of here is the essential outcome of your work. 

Two: Add implications, and don’t forget to specify who this might be relevant for: 

Students could be considered a specific subsample of the general population, but earlier research shows that the effect that exam stress has on their physical and mental health is comparable to the effects of other types of stress on individuals of other ages and occupations. Further research into practical ways of modulating not only one’s mental stress response but potentially also one’s brain activity (e.g., via neurofeedback training) are warranted.

This is a “research implication”, and it is nicely combined with a mention of a potential limitation of the study (the student sample) that turns out not to be a limitation after all (because earlier research suggests we can generalize to other populations). If there already is a lot of research on neurofeedback for stress control, by the way, then this should have been discussed in your discussion section earlier and you wouldn’t say such studies are “warranted” here but rather specify how your findings could inspire specific future experiments or how they should be implemented in existing applications. 

Three: The most important thing is that your conclusion paragraph accurately reflects the content of your paper. Compare it to your research paper title , your research paper abstract , and to your journal submission cover letter , in case you already have one—if these do not all tell the same story, then you need to go back to your paper, start again from the introduction section, and find out where you lost the logical thread. As always, consistency is key.    

Problems to Avoid When Writing a Conclusion 

  • Do not suddenly introduce new information that has never been mentioned before (unless you are writing an essay and opting for an externalizing conclusion, see above). The conclusion section is not where you want to surprise your readers, but the take-home message of what you have already presented.
  • Do not simply copy your abstract, the conclusion section of your abstract, or the first sentence of your introduction, and put it at the end of the discussion section. Even if these parts of your paper cover the same points, they should not be identical.
  • Do not start the conclusion with “In conclusion”. If it has its own section heading, that is redundant, and if it is the last paragraph of the discussion section, it is inelegant and also not really necessary. The reader expects you to wrap your work up in the last paragraph, so you don’t have to announce that. Just look at the above example to see how to start a conclusion in a natural way.
  • Do not forget what your research objectives were and how you initially formulated the statement of the problem in your introduction section. If your story/approach/conclusions changed because of methodological issues or information you were not aware of when you started, then make sure you go back to the beginning and adapt your entire story (not just the ending). 

Consider Receiving Academic Editing Services

When you have arrived at the conclusion of your paper, you might want to head over to Wordvice AI’s AI Writing Assistant to receive a free grammar check for any academic content. 

After drafting, you can also receive English editing and proofreading services , including paper editing services for your journal manuscript. If you need advice on how to write the other parts of your research paper , or on how to make a research paper outline if you are struggling with putting everything you did together, then head over to the Wordvice academic resources pages , where we have a lot more articles and videos for you.

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Finishing a research paper feels great, but getting to the end—especially the conclusion—can be a bit tricky. 

People often wonder, "How do I wrap up my findings nicely?" or "What tone should I use in the conclusion?" 

If you're dealing with these questions, you're not alone! Many researchers find writing a good conclusion a bit challenging since it's a crucial part that is meant to leave a strong impression on your readers.

No need to worry! 

In this guide, we'll show you how to write a conclusion that not only ties up your research paper neatly but also leaves a strong impression. We'll cover everything from summarizing effectively to creating the right feeling. 

So, let’s get started. 

Arrow Down

  • 1. What is a Research Paper Conclusion?
  • 2. How to Write a Research Paper Conclusion: 7 Steps
  • 3. Research Paper Conclusion Examples
  • 4. Things to Avoid While Writing the Research Paper Conclusion

What is a Research Paper Conclusion?

A research paper conclusion is like the final chapter of your paper. It's where you bring everything together and leave a lasting impression on your readers. 

In simple terms, it's the last part where you sum up what you found during your research and explain why it matters. 

The conclusion isn't just a summary; it's a chance to make your research memorable and show its importance.

Types of Research Paper Conclusions

When it comes to writing the conclusion of your research paper, there isn't a one-size-fits-all approach. Different types of research papers call for different types of conclusions. Here are some common types:

  • Summarizing Conclusion

This type recaps the key points and findings of your research. It's like giving your readers a quick overview of what you discovered without introducing new information. Summarizing conclusions works well for straightforward research papers.

  • Reflective Conclusion

A reflective conclusion allows you to share your personal thoughts on the research process, challenges faced, and lessons learned. It adds a human touch to your paper, giving readers insight into your journey as a researcher.

  • Open-ended Conclusion

Some research papers benefit from an open-ended conclusion that leaves room for further exploration. This type invites readers to think critically, ask questions, or even conduct additional research on the same topic.

How to Write a Research Paper Conclusion: 7 Steps

Writing an effective conclusion for your research paper involves more than just summarizing your findings. Follow these six essential steps to ensure your conclusion leaves a lasting impact:

Step 1: Restate the Research Problem

Start wrapping up your paper by going back to the main research question or issue you were investigating. 

Remind your readers about what you were trying to find out or understand. This gives your conclusion a clear connection to the original goal of your research, helping readers see the bigger picture.

Step 2: Revisit Your Thesis Statement

Go back to the main idea or argument you had in your paper—this is called your thesis statement. Double-check that your conclusion matches and supports what you wanted to prove or talk about in the beginning. 

This step is important because it keeps your conclusion connected to the main point of your research, making everything fit together nicely.

Step 3: Summarize Key Points

Give a short and clear recap of the most important things you found in your research. Keep it simple and stick to what you've already talked about—don't bring in new details now. 

The goal is to remind your readers of the important stuff you covered earlier. This helps to underline why your research is important and what you want them to take away from it.

Step 4: Discuss the Implications

Address the broader implications of your research. Explain why your findings matter and how they contribute to the existing body of knowledge in your field. 

Discuss the practical applications of your research and highlight any potential areas for further exploration.

Step 5: Connect with the Introduction

Create a seamless connection between your conclusion and the introduction. Referencing key elements from the introduction helps to create a cohesive narrative for your paper. 

This connection gives your research a sense of completeness and unity.

Step 6: Consider the "So What?" Factor

Ask yourself the question, "So what?" Why should readers care about your research? Clearly articulate the significance of your findings and their relevance to the broader academic or real-world context. 

Demonstrating the impact of your research adds depth to your conclusion.

Step 7: End with a Strong Closing Statement:

Conclude your research paper with a memorable closing statement. This could be a thought-provoking reflection, a call to action, or a suggestion for future research.

A strong closing leaves a lasting impression on your readers and emphasizes the importance of your work.

Research Paper Conclusion Examples

When it comes to writing a conclusion for your research paper, examining examples can offer valuable insights. Let’s take a look at this comprehensive example given below:

Still wondering how to write the conclusion for your research paper? Check out these examples for better understanding:

Conclusion For A Research Paper APA

Conclusion For A Research Paper Example Pdf

Conclusion For A Research Paper Pdf

Conclusion For A Research Paper Middle School

Conclusion For A Scientific Paper

Conclusion For A Research Paper Sample

Things to Avoid While Writing the Research Paper Conclusion

While crafting a conclusion for your research paper, it's crucial to steer clear of common pitfalls that can diminish the impact of your final remarks. 

Here are some things to avoid:

  • Repetition: Avoid rehashing the exact language used in the introduction or body of your paper. A conclusion should summarize key points without duplicating content.
  • Introducing New Information: Resist the temptation to introduce new ideas or data in the conclusion. This section is for summarizing existing content and reinforcing key findings.
  • Overly Complex Language: Keep your conclusion clear and accessible. Avoid introducing overly complex or technical language that might confuse your readers.
  • Lack of Connection to Introduction: Ensure that your conclusion ties back to the introduction. Failing to connect these sections can make your paper feel disjointed.
  • Vague Statements: Steer clear of vague statements that lack substance. Clearly articulate the significance of your findings and their broader implications.
  • Apologies or Excuses: Avoid including apologies or excuses for limitations in your research. While acknowledging limitations is important, the conclusion is not the place to dwell on them.
  • New Arguments or Debates: The conclusion is not the space to introduce new debates or arguments. Keep the focus on summarizing your research and its implications.
  • Abrupt Endings: A conclusion should not end abruptly. Instead, provide a thoughtful and well-rounded closing statement about the results of your study.

To sum it up, we've gone through important steps to make your research paper conclusion strong. We covered things like going back to your main question, talking about the most important points, and thinking about why your research matters in the real world.

Remember, a good ending is more than just a summary; it captures the heart of your research and answers the big "So what?" question. 

Remember, don't say the same things too much, don't add new details at the end, and keep your language simple!

If you ever need help with your academic writing, MyPerfectWords.com is here for you. Our expert writers are committed to helping you excel in your research papers and beyond. 

Take the next step towards academic success with MyPerfectWords.com, and hire our essay writing service today!

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Barbara P

Dr. Barbara is a highly experienced writer and author who holds a Ph.D. degree in public health from an Ivy League school. She has worked in the medical field for many years, conducting extensive research on various health topics. Her writing has been featured in several top-tier publications.

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  • How to conclude an essay | Interactive example

How to Conclude an Essay | Interactive Example

Published on January 24, 2019 by Shona McCombes . Revised on July 23, 2023.

The conclusion is the final paragraph of your essay . A strong conclusion aims to:

  • Tie together the essay’s main points
  • Show why your argument matters
  • Leave the reader with a strong impression

Your conclusion should give a sense of closure and completion to your argument, but also show what new questions or possibilities it has opened up.

This conclusion is taken from our annotated essay example , which discusses the history of the Braille system. Hover over each part to see why it’s effective.

Braille paved the way for dramatic cultural changes in the way blind people were treated and the opportunities available to them. Louis Braille’s innovation was to reimagine existing reading systems from a blind perspective, and the success of this invention required sighted teachers to adapt to their students’ reality instead of the other way around. In this sense, Braille helped drive broader social changes in the status of blindness. New accessibility tools provide practical advantages to those who need them, but they can also change the perspectives and attitudes of those who do not.

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Table of contents

Step 1: return to your thesis, step 2: review your main points, step 3: show why it matters, what shouldn’t go in the conclusion, more examples of essay conclusions, other interesting articles, frequently asked questions about writing an essay conclusion.

To begin your conclusion, signal that the essay is coming to an end by returning to your overall argument.

Don’t just repeat your thesis statement —instead, try to rephrase your argument in a way that shows how it has been developed since the introduction.

Prevent plagiarism. Run a free check.

Next, remind the reader of the main points that you used to support your argument.

Avoid simply summarizing each paragraph or repeating each point in order; try to bring your points together in a way that makes the connections between them clear. The conclusion is your final chance to show how all the paragraphs of your essay add up to a coherent whole.

To wrap up your conclusion, zoom out to a broader view of the topic and consider the implications of your argument. For example:

  • Does it contribute a new understanding of your topic?
  • Does it raise new questions for future study?
  • Does it lead to practical suggestions or predictions?
  • Can it be applied to different contexts?
  • Can it be connected to a broader debate or theme?

Whatever your essay is about, the conclusion should aim to emphasize the significance of your argument, whether that’s within your academic subject or in the wider world.

Try to end with a strong, decisive sentence, leaving the reader with a lingering sense of interest in your topic.

The easiest way to improve your conclusion is to eliminate these common mistakes.

Don’t include new evidence

Any evidence or analysis that is essential to supporting your thesis statement should appear in the main body of the essay.

The conclusion might include minor pieces of new information—for example, a sentence or two discussing broader implications, or a quotation that nicely summarizes your central point. But it shouldn’t introduce any major new sources or ideas that need further explanation to understand.

Don’t use “concluding phrases”

Avoid using obvious stock phrases to tell the reader what you’re doing:

  • “In conclusion…”
  • “To sum up…”

These phrases aren’t forbidden, but they can make your writing sound weak. By returning to your main argument, it will quickly become clear that you are concluding the essay—you shouldn’t have to spell it out.

Don’t undermine your argument

Avoid using apologetic phrases that sound uncertain or confused:

  • “This is just one approach among many.”
  • “There are good arguments on both sides of this issue.”
  • “There is no clear answer to this problem.”

Even if your essay has explored different points of view, your own position should be clear. There may be many possible approaches to the topic, but you want to leave the reader convinced that yours is the best one!

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This conclusion is taken from an argumentative essay about the internet’s impact on education. It acknowledges the opposing arguments while taking a clear, decisive position.

The internet has had a major positive impact on the world of education; occasional pitfalls aside, its value is evident in numerous applications. The future of teaching lies in the possibilities the internet opens up for communication, research, and interactivity. As the popularity of distance learning shows, students value the flexibility and accessibility offered by digital education, and educators should fully embrace these advantages. The internet’s dangers, real and imaginary, have been documented exhaustively by skeptics, but the internet is here to stay; it is time to focus seriously on its potential for good.

This conclusion is taken from a short expository essay that explains the invention of the printing press and its effects on European society. It focuses on giving a clear, concise overview of what was covered in the essay.

The invention of the printing press was important not only in terms of its immediate cultural and economic effects, but also in terms of its major impact on politics and religion across Europe. In the century following the invention of the printing press, the relatively stationary intellectual atmosphere of the Middle Ages gave way to the social upheavals of the Reformation and the Renaissance. A single technological innovation had contributed to the total reshaping of the continent.

This conclusion is taken from a literary analysis essay about Mary Shelley’s Frankenstein . It summarizes what the essay’s analysis achieved and emphasizes its originality.

By tracing the depiction of Frankenstein through the novel’s three volumes, I have demonstrated how the narrative structure shifts our perception of the character. While the Frankenstein of the first volume is depicted as having innocent intentions, the second and third volumes—first in the creature’s accusatory voice, and then in his own voice—increasingly undermine him, causing him to appear alternately ridiculous and vindictive. Far from the one-dimensional villain he is often taken to be, the character of Frankenstein is compelling because of the dynamic narrative frame in which he is placed. In this frame, Frankenstein’s narrative self-presentation responds to the images of him we see from others’ perspectives. This conclusion sheds new light on the novel, foregrounding Shelley’s unique layering of narrative perspectives and its importance for the depiction of character.

If you want to know more about AI tools , college essays , or fallacies make sure to check out some of our other articles with explanations and examples or go directly to our tools!

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Your essay’s conclusion should contain:

  • A rephrased version of your overall thesis
  • A brief review of the key points you made in the main body
  • An indication of why your argument matters

The conclusion may also reflect on the broader implications of your argument, showing how your ideas could applied to other contexts or debates.

For a stronger conclusion paragraph, avoid including:

  • Important evidence or analysis that wasn’t mentioned in the main body
  • Generic concluding phrases (e.g. “In conclusion…”)
  • Weak statements that undermine your argument (e.g. “There are good points on both sides of this issue.”)

Your conclusion should leave the reader with a strong, decisive impression of your work.

The conclusion paragraph of an essay is usually shorter than the introduction . As a rule, it shouldn’t take up more than 10–15% of the text.

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The conclusion is intended to help the reader understand why your research should matter to them after they have finished reading the paper. A conclusion is not merely a summary of the main topics covered or a re-statement of your research problem, but a synthesis of key points and, if applicable, where you recommend new areas for future research. For most college-level research papers, one or two well-developed paragraphs is sufficient for a conclusion, although in some cases, more paragraphs may be required in summarizing key findings and their significance.

Conclusions. The Writing Center. University of North Carolina; Conclusions. The Writing Lab and The OWL. Purdue University.

Importance of a Good Conclusion

A well-written conclusion provides you with important opportunities to demonstrate to the reader your understanding of the research problem. These include:

  • Presenting the last word on the issues you raised in your paper . Just as the introduction gives a first impression to your reader, the conclusion offers a chance to leave a lasting impression. Do this, for example, by highlighting key findings in your analysis that advance new understanding about the research problem, that are unusual or unexpected, or that have important implications applied to practice.
  • Summarizing your thoughts and conveying the larger significance of your study . The conclusion is an opportunity to succinctly re-emphasize  the "So What?" question by placing the study within the context of how your research advances past research about the topic.
  • Identifying how a gap in the literature has been addressed . The conclusion can be where you describe how a previously identified gap in the literature [described in your literature review section] has been filled by your research.
  • Demonstrating the importance of your ideas . Don't be shy. The conclusion offers you the opportunity to elaborate on the impact and significance of your findings. This is particularly important if your study approached examining the research problem from an unusual or innovative perspective.
  • Introducing possible new or expanded ways of thinking about the research problem . This does not refer to introducing new information [which should be avoided], but to offer new insight and creative approaches for framing or contextualizing the research problem based on the results of your study.

Bunton, David. “The Structure of PhD Conclusion Chapters.” Journal of English for Academic Purposes 4 (July 2005): 207–224; Conclusions. The Writing Center. University of North Carolina; Kretchmer, Paul. Twelve Steps to Writing an Effective Conclusion. San Francisco Edit, 2003-2008; Conclusions. The Writing Lab and The OWL. Purdue University; Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8.

Structure and Writing Style

I.  General Rules

The function of your paper's conclusion is to restate the main argument . It reminds the reader of the strengths of your main argument(s) and reiterates the most important evidence supporting those argument(s). Do this by stating clearly the context, background, and necessity of pursuing the research problem you investigated in relation to an issue, controversy, or a gap found in the literature. Make sure, however, that your conclusion is not simply a repetitive summary of the findings. This reduces the impact of the argument(s) you have developed in your essay.

When writing the conclusion to your paper, follow these general rules:

  • Present your conclusions in clear, simple language. Re-state the purpose of your study, then describe how your findings differ or support those of other studies and why [i.e., what were the unique or new contributions your study made to the overall research about your topic?].
  • Do not simply reiterate your findings or the discussion of your results. Provide a synthesis of arguments presented in the paper to show how these converge to address the research problem and the overall objectives of your study.
  • Indicate opportunities for future research if you haven't already done so in the discussion section of your paper. Highlighting the need for further research provides the reader with evidence that you have an in-depth awareness of the research problem and that further investigations should take place.

Consider the following points to help ensure your conclusion is presented well:

  • If the argument or purpose of your paper is complex, you may need to summarize the argument for your reader.
  • If, prior to your conclusion, you have not yet explained the significance of your findings or if you are proceeding inductively, use the end of your paper to describe your main points and explain their significance.
  • Move from a detailed to a general level of consideration that returns the topic to the context provided by the introduction or within a new context that emerges from the data. 

The conclusion also provides a place for you to persuasively and succinctly restate the research problem, given that the reader has now been presented with all the information about the topic . Depending on the discipline you are writing in, the concluding paragraph may contain your reflections on the evidence presented. However, the nature of being introspective about the research you have conducted will depend on the topic and whether your professor wants you to express your observations in this way.

NOTE : If asked to think introspectively about the topics, do not delve into idle speculation. Being introspective means looking within yourself as an author to try and understand an issue more deeply, not to guess at possible outcomes or make up scenarios not supported by the evidence.

II.  Developing a Compelling Conclusion

Although an effective conclusion needs to be clear and succinct, it does not need to be written passively or lack a compelling narrative. Strategies to help you move beyond merely summarizing the key points of your research paper may include any of the following strategies:

  • If your essay deals with a critical, contemporary problem, warn readers of the possible consequences of not attending to the problem proactively.
  • Recommend a specific course or courses of action that, if adopted, could address a specific problem in practice or in the development of new knowledge.
  • Cite a relevant quotation or expert opinion already noted in your paper in order to lend authority and support to the conclusion(s) you have reached [a good place to look is research from your literature review].
  • Explain the consequences of your research in a way that elicits action or demonstrates urgency in seeking change.
  • Restate a key statistic, fact, or visual image to emphasize the most important finding of your paper.
  • If your discipline encourages personal reflection, illustrate your concluding point by drawing from your own life experiences.
  • Return to an anecdote, an example, or a quotation that you presented in your introduction, but add further insight derived from the findings of your study; use your interpretation of results to recast it in new or important ways.
  • Provide a "take-home" message in the form of a succinct, declarative statement that you want the reader to remember about your study.

III. Problems to Avoid

Failure to be concise Your conclusion section should be concise and to the point. Conclusions that are too lengthy often have unnecessary information in them. The conclusion is not the place for details about your methodology or results. Although you should give a summary of what was learned from your research, this summary should be relatively brief, since the emphasis in the conclusion is on the implications, evaluations, insights, and other forms of analysis that you make. Strategies for writing concisely can be found here .

Failure to comment on larger, more significant issues In the introduction, your task was to move from the general [the field of study] to the specific [the research problem]. However, in the conclusion, your task is to move from a specific discussion [your research problem] back to a general discussion [i.e., how your research contributes new understanding or fills an important gap in the literature]. In short, the conclusion is where you should place your research within a larger context [visualize your paper as an hourglass--start with a broad introduction and review of the literature, move to the specific analysis and discussion, conclude with a broad summary of the study's implications and significance].

Failure to reveal problems and negative results Negative aspects of the research process should never be ignored. These are problems, deficiencies, or challenges encountered during your study should be summarized as a way of qualifying your overall conclusions. If you encountered negative or unintended results [i.e., findings that are validated outside the research context in which they were generated], you must report them in the results section and discuss their implications in the discussion section of your paper. In the conclusion, use your summary of the negative results as an opportunity to explain their possible significance and/or how they may form the basis for future research.

Failure to provide a clear summary of what was learned In order to be able to discuss how your research fits within your field of study [and possibly the world at large], you need to summarize briefly and succinctly how it contributes to new knowledge or a new understanding about the research problem. This element of your conclusion may be only a few sentences long.

Failure to match the objectives of your research Often research objectives in the social sciences change while the research is being carried out. This is not a problem unless you forget to go back and refine the original objectives in your introduction. As these changes emerge they must be documented so that they accurately reflect what you were trying to accomplish in your research [not what you thought you might accomplish when you began].

Resist the urge to apologize If you've immersed yourself in studying the research problem, you presumably should know a good deal about it [perhaps even more than your professor!]. Nevertheless, by the time you have finished writing, you may be having some doubts about what you have produced. Repress those doubts! Don't undermine your authority by saying something like, "This is just one approach to examining this problem; there may be other, much better approaches that...." The overall tone of your conclusion should convey confidence to the reader.

Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8; Concluding Paragraphs. College Writing Center at Meramec. St. Louis Community College; Conclusions. The Writing Center. University of North Carolina; Conclusions. The Writing Lab and The OWL. Purdue University; Freedman, Leora  and Jerry Plotnick. Introductions and Conclusions. The Lab Report. University College Writing Centre. University of Toronto; Leibensperger, Summer. Draft Your Conclusion. Academic Center, the University of Houston-Victoria, 2003; Make Your Last Words Count. The Writer’s Handbook. Writing Center. University of Wisconsin Madison; Miquel, Fuster-Marquez and Carmen Gregori-Signes. “Chapter Six: ‘Last but Not Least:’ Writing the Conclusion of Your Paper.” In Writing an Applied Linguistics Thesis or Dissertation: A Guide to Presenting Empirical Research . John Bitchener, editor. (Basingstoke,UK: Palgrave Macmillan, 2010), pp. 93-105; Tips for Writing a Good Conclusion. Writing@CSU. Colorado State University; Kretchmer, Paul. Twelve Steps to Writing an Effective Conclusion. San Francisco Edit, 2003-2008; Writing Conclusions. Writing Tutorial Services, Center for Innovative Teaching and Learning. Indiana University; Writing: Considering Structure and Organization. Institute for Writing Rhetoric. Dartmouth College.

Writing Tip

Don't Belabor the Obvious!

Avoid phrases like "in conclusion...," "in summary...," or "in closing...." These phrases can be useful, even welcome, in oral presentations. But readers can see by the tell-tale section heading and number of pages remaining to read, when an essay is about to end. You'll irritate your readers if you belabor the obvious.

Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8.

Another Writing Tip

New Insight, Not New Information!

Don't surprise the reader with new information in your conclusion that was never referenced anywhere else in the paper and, as such, the conclusion rarely has citations to sources. If you have new information to present, add it to the discussion or other appropriate section of the paper. Note that, although no actual new information is introduced, the conclusion, along with the discussion section, is where you offer your most "original" contributions in the paper; the conclusion is where you describe the value of your research, demonstrate that you understand the material that you’ve presented, and locate your findings within the larger context of scholarship on the topic, including describing how your research contributes new insights or valuable insight to that scholarship.

Assan, Joseph. "Writing the Conclusion Chapter: The Good, the Bad and the Missing." Liverpool: Development Studies Association (2009): 1-8; Conclusions. The Writing Center. University of North Carolina.

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In a short paper—even a research paper—you don’t need to provide an exhaustive summary as part of your conclusion. But you do need to make some kind of transition between your final body paragraph and your concluding paragraph. This may come in the form of a few sentences of summary. Or it may come in the form of a sentence that brings your readers back to your thesis or main idea and reminds your readers where you began and how far you have traveled.

So, for example, in a paper about the relationship between ADHD and rejection sensitivity, Vanessa Roser begins by introducing readers to the fact that researchers have studied the relationship between the two conditions and then provides her explanation of that relationship. Here’s her thesis: “While socialization may indeed be an important factor in RS, I argue that individuals with ADHD may also possess a neurological predisposition to RS that is exacerbated by the differing executive and emotional regulation characteristic of ADHD.”

In her final paragraph, Roser reminds us of where she started by echoing her thesis: “This literature demonstrates that, as with many other conditions, ADHD and RS share a delicately intertwined pattern of neurological similarities that is rooted in the innate biology of an individual’s mind, a connection that cannot be explained in full by the behavioral mediation hypothesis.”  

Highlight the “so what”  

At the beginning of your paper, you explain to your readers what’s at stake—why they should care about the argument you’re making. In your conclusion, you can bring readers back to those stakes by reminding them why your argument is important in the first place. You can also draft a few sentences that put those stakes into a new or broader context.

In the conclusion to her paper about ADHD and RS, Roser echoes the stakes she established in her introduction—that research into connections between ADHD and RS has led to contradictory results, raising questions about the “behavioral mediation hypothesis.”

She writes, “as with many other conditions, ADHD and RS share a delicately intertwined pattern of neurological similarities that is rooted in the innate biology of an individual’s mind, a connection that cannot be explained in full by the behavioral mediation hypothesis.”  

Leave your readers with the “now what”  

After the “what” and the “so what,” you should leave your reader with some final thoughts. If you have written a strong introduction, your readers will know why you have been arguing what you have been arguing—and why they should care. And if you’ve made a good case for your thesis, then your readers should be in a position to see things in a new way, understand new questions, or be ready for something that they weren’t ready for before they read your paper.

In her conclusion, Roser offers two “now what” statements. First, she explains that it is important to recognize that the flawed behavioral mediation hypothesis “seems to place a degree of fault on the individual. It implies that individuals with ADHD must have elicited such frequent or intense rejection by virtue of their inadequate social skills, erasing the possibility that they may simply possess a natural sensitivity to emotion.” She then highlights the broader implications for treatment of people with ADHD, noting that recognizing the actual connection between rejection sensitivity and ADHD “has profound implications for understanding how individuals with ADHD might best be treated in educational settings, by counselors, family, peers, or even society as a whole.”

To find your own “now what” for your essay’s conclusion, try asking yourself these questions:

  • What can my readers now understand, see in a new light, or grapple with that they would not have understood in the same way before reading my paper? Are we a step closer to understanding a larger phenomenon or to understanding why what was at stake is so important?  
  • What questions can I now raise that would not have made sense at the beginning of my paper? Questions for further research? Other ways that this topic could be approached?  
  • Are there other applications for my research? Could my questions be asked about different data in a different context? Could I use my methods to answer a different question?  
  • What action should be taken in light of this argument? What action do I predict will be taken or could lead to a solution?  
  • What larger context might my argument be a part of?  

What to avoid in your conclusion  

  • a complete restatement of all that you have said in your paper.  
  • a substantial counterargument that you do not have space to refute; you should introduce counterarguments before your conclusion.  
  • an apology for what you have not said. If you need to explain the scope of your paper, you should do this sooner—but don’t apologize for what you have not discussed in your paper.  
  • fake transitions like “in conclusion” that are followed by sentences that aren’t actually conclusions. (“In conclusion, I have now demonstrated that my thesis is correct.”)
  • picture_as_pdf Conclusions

How to Write a Conclusion for a Research Paper: Examples & Tips

You might be wondering about how to write a conclusion paragraph for a research paper. It may seem like your readers should understand your main arguments by the end, so there is no need for it. However, there are several aspects that prove the importance of a conclusion section in research.

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Its first and primary function is, of course, a summary of all the main ideas and evidence in the paper. Sometimes research can be quite lengthy so putting all the thoughts you want to share in one place is very handy. Moreover, the conclusion shows how important your work is and suggests new ways of looking at the problem.

Our guide and research paper conclusion example are here to help you with your assignment!

  • ❗ Importance of a Conclusion
  • 👣 Writing Steps
  • 📑 Conclusion Types & Examples
  • ❌ Common Mistakes

🔗 References

❗ importance of a conclusion in a research paper.

A conclusion intends to remind the readers about the main arguments and findings of the whole paper. However, it also highlights the significance of the work. Both these functions help create a long-lasting, memorable impression from your research paper , so always include this part and try to think of the ways to make it even more effective.

Since having a strong concluding paragraph is so crucial for the overall success, you might want to check out a step-by-step guide on writing it. It can assure you don’t miss any vital moments. Moreover, you need to find out what conclusion type would be the best!

👣 How to Write a Conclusion for a Research Paper Step by Step

Below are only a few simple steps that can allow you to write the most persuasive research paper.

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📑 Types & Examples of Research Paper Conclusions

You may be assigned to write a persuasive or argumentative paper. Or your professor might ask you to develop an analytical or comparative research paper.

Should you write their conclusions in the same way? The answer is “No”. When you write different types of assignments, you need different conclusions.

Argumentative Research Paper Conclusion

To write an excellent argumentative paper conclusion, you need to highlight the most persuasive and strong arguments you have — no need to add many details. In addition, don’t forget you should include the essential components of the conclusion, such as paraphrasing your thesis statement, which points out your opinion on the chosen topic. If you used a strong thesis statement generator , it won’t be hard to do.

Argumentative Research Paper Conclusion Example

Right now, Earth is facing the issue of the sixth extinction, which causes numerous species to fade every day. There are at least three ideas people might use to prevent their total extinction in the next fifty years. More ways of recycling, new approaches to plastic production, and conservation of species could be life-saving.

Analytical Research Paper Conclusion

First, you should restate your thesis statement and summarize the critical points of your arguments. The main difference between the analytical research paper conclusion and other types is that there should definitely be a highlight of a broader context. It means you can add some meaning to the findings.

Analytical Research Paper Conclusion Example

Elon Musk has made a revolution in the way we pay, drive, and even fly. His ideas come only from the desire to make things easier, but eventually, they change the world. Musk first thought about PayPal when he was a student, and now it’s one of the most popular online payment systems. The same with Tesla cars.

Comparative Research Paper Conclusion

An effective comparative paper conclusion requires some analytical skills. You need to be very careful in looking through facts to clearly formulate your findings. Moreover, the sources need to be trustworthy. And, as usual, you need to add a paraphrased thesis statement and a few words about the importance of your study research.

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Comparative Research Paper Conclusion Example

Electric cars are proven to be more efficient and effective than gas cars. Not only do they produce fewer emissions, but the drivers reach their destination point faster. Moreover, gas cars are more expensive to maintain. It all derives from the specifics of the electric cars’ engines, which are much simpler.

❌ Common Mistakes You Should Avoid

Are you ready to pass your writing? Wait! Have you checked it for the most common mistakes? If no, below are several general errors you should avoid.

  • The Conclusion – Organizing Academic Research Papers
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  • How to Structure & Organize Your Paper
  • Writing Conclusions: Writing Guides
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Very, very useful website for students. I appreciate people who kindly share brilliant writing tips with others! Thanks a lot!

Great article! It helped me to complete my research papers conclusion that was a real nightmare for me! Thank you so much for it!

I was on my way to completing my research paper and going to finish the conclusion by copy-pasting the introduction. Thank God, I wanted to read some articles on conclusion writing. After reading your post on this question, I completed my conclusion following your instructions. Believe my research paper is worth an excellent mark!

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In response to a comment from Lynn: Thank you for noting this unfortunate mistake in the text. Obviously, we spend a lot of time compiling, writing, and editing materials, but there’s always room for a simple human error! Thanks again, and have a great day! 🙂

This helped a lot, thanks =)

This helped me so much! Thank you!

This paper is in the following e-collection/theme issue:

Published on 28.3.2024 in Vol 26 (2024)

Development and External Validation of Machine Learning Models for Diabetic Microvascular Complications: Cross-Sectional Study With Metabolites

Authors of this article:

Author Orcid Image

Original Paper

  • Feng He 1, 2 , MSc   ; 
  • Clarissa Ng Yin Ling 1 , MD   ; 
  • Simon Nusinovici 1, 3 , PhD   ; 
  • Ching-Yu Cheng 1, 3 , PhD   ; 
  • Tien Yin Wong 1, 3 , PhD   ; 
  • Jialiang Li 2 * , PhD   ; 
  • Charumathi Sabanayagam 1, 3 * , PhD  

1 Singapore Eye Research Institute, Singapore National Eye Centre, Singapore, Singapore

2 Department of Statistics and Data Science, National University of Singapore, Singapore, Singapore

3 Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Medical School, Singapore, Singapore

*these authors contributed equally

Corresponding Author:

Charumathi Sabanayagam, PhD

Singapore Eye Research Institute

Singapore National Eye Centre

The Academia, 20 College Road, Discovery Tower Level 6

Singapore, 169856

Phone: 65 6576 7286

Fax:65 6225 2568

Email: [email protected]

Background: Diabetic kidney disease (DKD) and diabetic retinopathy (DR) are major diabetic microvascular complications, contributing significantly to morbidity, disability, and mortality worldwide. The kidney and the eye, having similar microvascular structures and physiological and pathogenic features, may experience similar metabolic changes in diabetes.

Objective: This study aimed to use machine learning (ML) methods integrated with metabolic data to identify biomarkers associated with DKD and DR in a multiethnic Asian population with diabetes, as well as to improve the performance of DKD and DR detection models beyond traditional risk factors.

Methods: We used ML algorithms (logistic regression [LR] with Least Absolute Shrinkage and Selection Operator and gradient-boosting decision tree) to analyze 2772 adults with diabetes from the Singapore Epidemiology of Eye Diseases study, a population-based cross-sectional study conducted in Singapore (2004-2011). From 220 circulating metabolites and 19 risk factors, we selected the most important variables associated with DKD (defined as an estimated glomerular filtration rate <60 mL/min/1.73 m 2 ) and DR (defined as an Early Treatment Diabetic Retinopathy Study severity level ≥20). DKD and DR detection models were developed based on the variable selection results and externally validated on a sample of 5843 participants with diabetes from the UK biobank (2007-2010). Machine-learned model performance (area under the receiver operating characteristic curve [AUC] with 95% CI, sensitivity, and specificity) was compared to that of traditional LR adjusted for age, sex, diabetes duration, hemoglobin A 1c , systolic blood pressure, and BMI.

Results: Singapore Epidemiology of Eye Diseases participants had a median age of 61.7 (IQR 53.5-69.4) years, with 49.1% (1361/2772) being women, 20.2% (555/2753) having DKD, and 25.4% (685/2693) having DR. UK biobank participants had a median age of 61.0 (IQR 55.0-65.0) years, with 35.8% (2090/5843) being women, 6.7% (374/5570) having DKD, and 6.1% (355/5843) having DR. The ML algorithms identified diabetes duration, insulin usage, age, and tyrosine as the most important factors of both DKD and DR. DKD was additionally associated with cardiovascular disease history, antihypertensive medication use, and 3 metabolites (lactate, citrate, and cholesterol esters to total lipids ratio in intermediate-density lipoprotein), while DR was additionally associated with hemoglobin A 1c , blood glucose, pulse pressure, and alanine. Machine-learned models for DKD and DR detection outperformed traditional LR models in both internal (AUC 0.838 vs 0.743 for DKD and 0.790 vs 0.764 for DR) and external validation (AUC 0.791 vs 0.691 for DKD and 0.778 vs 0.760 for DR).

Conclusions: This study highlighted diabetes duration, insulin usage, age, and circulating tyrosine as important factors in detecting DKD and DR. The integration of ML with biomedical big data enables biomarker discovery and improves disease detection beyond traditional risk factors.

Introduction

Diabetes is a complex metabolic disorder and a major global health problem of our time. In 2021, it affected 536.6 million adults worldwide, with a projected surge to 783.2 million by 2045 [ 1 ]. With the rapidly growing population with diabetes and the greater longevity over time, the burden of associated complications is expected to increase in parallel [ 2 ]. Among these, diabetic kidney disease (DKD) was estimated to develop in around 40% of the population with diabetes [ 3 ], while diabetic retinopathy (DR) would manifest in approximately 35% [ 4 ]. Left undetected and untreated, these microvascular complications could substantially elevate the risk of cardiovascular disease (CVD), end-stage renal disease, and permanent vision loss, resulting in compromised quality of life and shortened life expectancy [ 2 , 5 , 6 ]. Yet, timely and accurate diagnosis of DKD and DR remains a challenge because of their asymptomatic progression in the early stages [ 7 ]. Although factors such as age, sex, diabetes duration, hemoglobin A 1c (HbA 1c ) %, systolic blood pressure (SBP), and BMI have been identified as the risk contributors for DKD and DR, they offer only partial insights into the variability of risk among individuals.

To enable risk prediction and exploration of the underlying metabolic pathways, metabolomics has been increasingly used for biomarker discovery in diabetes and its complications [ 7 - 10 ]. Abnormalities in amino acids and lipids metabolism have been linked to both DKD and DR [ 7 ]. Given the similar microvascular structure, physiology, and pathogenic features of the kidney and the eye, these common metabolic traits may indicate biochemical pathways shared by the 2 complications, or both being manifestations of a latent systematic condition [ 11 ]. However, to the best of our knowledge, investigations into the commonalities and differences between DKD and DR in terms of their metabolic basis have been somewhat scarce [ 7 , 12 ]. Moreover, prior research was often limited by inadequate sample sizes, lack of replication, and restricted data analysis methods [ 13 ].

In this study, we aimed to fill these gaps by analyzing 220 circulating metabolites and 19 established risk factors as predictors of prevalent DKD and DR in a retrospective Asian adult population with diabetes. We used 2 machine learning (ML) algorithms, logistic regression (LR) with Least Absolute Shrinkage and Selection Operator (LASSO [ 14 ]), and a gradient-boosting decision tree (GBDT [ 15 ]). Compared with traditional statistical methods, these ML algorithms excel in handling high-dimensional data with complex relationships and can quantify the relative contribution of individual variables through variable importance scores. Based on these scores, we selected the top variables to develop ML models for DKD and DR detection and validated them externally in UK biobank (UKBB [ 16 ]) data. Additionally, we developed reference models using LR adjusted for traditional risk factors. All models were evaluated using the area under the receiver operating characteristic curve (AUC) with 95% CI, sensitivity, and specificity. Finally, we discussed the potential role of the selected metabolites in DKD and DR with reference to previous studies.

We derived this study’s data from the Singapore Epidemiology of Eye Diseases study (SEED [ 17 ]), a population-based cross-sectional study conducted in Singapore from 2004 to 2011. Detailed methodology has been reported elsewhere [ 17 ]. In brief, we recruited 10,033 adults aged 40-80 years using age-stratified random sampling. Participants completed interviewer-administered questionnaires, underwent ocular examinations, and provided samples for biochemical laboratory tests. The cohort included 3280 Malay (2004-2006, response rate 78.7%), 3400 Indian (2007-2009, response rate 75.6%), and 3353 Chinese (2009-2011, response rate 72.8%) individuals.

For external validation, we used data from UKBB, an open-access resource of prospective data set collected in the UK from 2007 to 2010, with over 500,000 participants recruited between the ages of 40 to 69 [ 16 ].

Ethical Considerations

Both SEED and UKBB studies were conducted per the Declaration of Helsinki. Ethical approval was obtained from the SingHealth Institutional Review Board for SEED (2018/2717, 2018/2921, 2018/2006, 2018/2594, 2018/2570, 2015/2279, and 2012/487/A) and from North West Multi-centre Research Ethics Committee for UKBB (21/NW/0157). Written informed consent was provided by all participants during the primary data collection. Due to the retrospective nature of our study and the use of deidentified health information, the SingHealth Institutional Review Board approved this study without requiring additional patient consent.

Definition of Outcome and Variables

In SEED, diabetes was defined as meeting any of the following criteria: HbA 1c % >6.5, random blood glucose >11.1 mmol/L, self-reported physician-diagnosed diabetes, or the use of antidiabetic medication including insulin. In UKBB, we applied the same definition but additionally included individuals with DR if the aforementioned variables were missing.

DKD was defined as an estimated glomerular filtration rate (eGFR) <60 mL/min/1.73m 2 in people with diabetes. The eGFR values were calculated from blood creatinine concentrations using the chronic kidney disease epidemiology collaboration equation [ 18 ].

For SEED participants with diabetes, DR severity in each eye was graded from fundus photographs by certified ophthalmic graders according to the standard protocol of Early Treatment Diabetic Retinopathy Study (ETDRS) [ 2 , 19 ] with 6 stages: no DR (level 10-20), minimal (level 20), mild (level 35), moderate (levels 43 to 47), severe (level 53), and proliferative DR (levels >60). We defined the DR outcome as having an ETDRS level ≥20 in at least one eye (ie, any DR). In UKBB, DR severity was not graded. Therefore, we identified DR cases based on the International Classification of Diseases, Tenth Revision (ICD-10) code “H36.0” in their health-related outcomes (UKBB Data-Field: 41270) [ 16 ]. Supplementary analysis in SEED also considered moderate or worse DR (level >43 in at least one eye) as an additional outcome.

We considered a total of 239 variables for biomarker selection ( Multimedia Appendix 1 ). Among these, 19 variables were identified through a literature review, comprising 6 traditional risk factors (age, sex, duration of diabetes, HbA 1c %, SBP, and BMI), and 13 extended risk factors related to lifestyle (alcohol consumption and smoking), medication use (insulin, anticholesterol, and antihypertensive medication), clinic or biochemistry (diastolic blood pressure [DBP], pulse pressure [PP], random blood glucose, total cholesterol, high-density lipoprotein [HDL] cholesterol, and low-density lipoprotein cholesterol), and comorbidity conditions (hypertension and history of CVD). Hypertension in both cohorts was defined as self-reported physician-diagnosed hypertension, SBP >140 mm Hg, DBP >80 mm Hg, or the use of antihypertensive medication.

Using nuclear magnetic resonance techniques (Nightingale Health), we quantified the concentration of 228 circulating metabolites from patients’ blood samples. Of these, glycerol, pyruvate, and glutamine were not available for Malay individuals, creatinine was used in eGFR calculation and DKD outcome definition, while 4 metabolites (total, HDL, low-density lipoprotein cholesterols, and random blood glucose) were duplicated with those measured in biochemistry tests. Therefore, these variables were excluded from the analysis, leaving us with 220 metabolites from 15 categories (amino acids, apolipoproteins, cholesterol, cholesterol esters, fatty acids, fluid balance, free cholesterols, glycolysis-related metabolites, inflammation, ketone bodies, triglycerides, lipoprotein particle sizes, lipoprotein subclasses, lipoprotein lipid ratios, and other lipids).

Statistical Analysis

Inclusion and exclusion criteria.

From the initial SEED data set of 10,033 participants, we excluded those free of diabetes (n=7069), missing metabolomics profiles (n=179), or missing more than 10% (n=13) of the data, to obtain a final study population of 2772 individuals. Similarly, we identified 5843 UKBB participants eligible for external validation after data cleaning ( Figure 1 ).

sample conclusion on research

Descriptive Statistics

We categorized SEED participants into 4 groups based on their DKD and DR status, and summarized group characteristics as number (%), mean (SD), or median (IQR) as appropriate for each variable. Differences among groups were assessed using 2-sided P values derived from Pearson χ 2 tests or Fisher exact test for categorical variables, and Kruskal-Wallis rank sum tests for numeric variables. We also evaluated the interpopulation differences between SEED and UKBB using Pearson χ 2 tests and Mann-Whitney U tests as appropriate for each variable. Some subcategories may not add up due to the presence of missing data.

ML Algorithms

We used LASSO [ 14 ] and GBDT [ 15 ] to identify the key biomarkers and develop disease detection models for DR and DKD. LASSO is an extension of traditional LR that does not require the independence of variables. Therefore, LASSO is suitable for high-dimensional data sets where issues such as multicollinearity often arise. During parameter optimization, LASSO automatically shrinks the coefficients of the less-important variables to zero, while retaining nonzero coefficients for the important ones to achieve biomarker selection. Its strengths include relatively straightforward computation and parameter tuning compared to other ML algorithms. However, its scope is limited to examining only the linear associations between continuous variables and the log-odds. To account for possible nonlinear effects and variable interactions, we additionally implemented the GBDT algorithm, which constructs a sequence of interdependent decision trees to collectively make a decision. During the process, the algorithm assesses each variable’s contribution to minimizing prediction errors, returning a score for its relative influence. We then applied a predefined threshold to select the most influential variables for disease detection based on their scores in each selection round. GBDT is known for its adaptability to various data distributions with generally strong performances, yet its hyper-parameter tuning and computation can be rather time-consuming, with a higher chance of overfitting, and less transparency compared to LASSO.

Model Development and Validation

In SEED, we handled missing data by excluding variables and participants with high levels of missing information, yielding a final data set with each variable containing less than 6% (161/2772) missing data and a participant-level missing data percentage of 10% or less (23/239, Multimedia Appendix 2 ). Following this, missing data imputation was carried out by using mean values for numeric variables, and modes for categorical variables as appropriate. To mitigate selection bias arising from data division into training and validation sets, we averaged the results over 200 random repeats of 5-fold cross-validation. In each repeat, the imputed SEED data set was randomly divided into 5 subsets (ie, folds) of equal sample size by stratified sampling to ensure consistent case rates. Each fold (553/2772, 20% of data) took its turn as the validation set, while the remaining 4 folds (2219/2772, 80% of data) were used for model training and variable selection. From 200 replicates we generated 1000 sets of variable selection results, based on which we quantified each variable’s contribution to the model’s performance by calculating a variable importance score, as the variable’s selection frequency during the repeated cross-validation process.

Next, we arranged the variables in descending order of their selection frequencies and included only those selected 990 times or more (ie, selection frequency 0.990) in the final model. To evaluate the performance of these new models, we performed another 200 random repeats of 5-fold cross-validation but used only the complete cases (ie, no missing data imputation). The final machine-learned models were compared with the multivariate LR models adjusted for the 6 established risk factors including age, sex, diabetes duration, HbA 1c %, SBP, and BMI. The performance of these models was evaluated in internal and external validation using AUC with 95% CI, sensitivity, and specificity. We reported sensitivity and specificity at the optimal threshold, where sensitivity equals specificity. Additionally, in the context of DR and DKD detection, where the cost of false negatives is generally higher than that of false positives, we prioritized higher sensitivity over higher specificity by setting the probability threshold at 0.8 sensitivity to compare the specificity. The AUC difference between machine-learned models and the traditional model was evaluated using the test by DeLong et al [ 20 ]. For the supplementary analysis, we examined the importance of the top machine-learned variables within nested models, to check for any marginal increase in AUC with additional variables. We also calculated the AUC for ML models with all the variables.

Metabolites Selection

Finally, we focused on the metabolites that were consistently selected by both ML algorithms. We quantified their associations with DKD and DR respectively, using odds ratios (OR) per SD increment with 95% CI, and P values from multivariate LR models adjusted for age, sex, diabetes duration, insulin use, HbA 1c %, PP, BMI, cholesterol, and HDL cholesterol.

We conducted all the analyses in R version 4.0.2. (R Foundation) and defined statistical significance as P <.05.

Population Characteristics

Figure 1 illustrates the data cleaning and analysis workflow. In the SEED population with diabetes, 2674 people had information on both disease outcomes ( Multimedia Appendix 3 ). Of these, 1657 (62%) had neither DKD nor DR, 338 (12.6%) had DKD but not DR, 496 (18.5%) had DR but not DKD, and 183 (6.8%) had both DKD and DR. People with DKD, regardless of their DR status, tended to be older, with higher PP, higher SBP, and lower DBP. They also had higher levels of HDL cholesterol, more history of hypertension and CVD, and were more likely to have used anticholesterol medication and antihypertensive medication. However, they had a lower smoking rate and reported less alcohol consumption than those without DKD. People with DR, regardless of their DKD status, tended to have a longer duration of diabetes, with the use of antidiabetic medication and insulin, higher HbA 1c %, and random blood glucose levels. They also had a lower BMI and lower total cholesterol levels. For people with both complications, the abovementioned characteristics further differed from those with neither complication. However, in terms of sex distribution, no significant difference was observed ( P =.09).

We also found differences between SEED and UKBB in terms of demographics, lifestyle factors, biochemical laboratory results, and medical history ( Multimedia Appendix 4 ). In particular, UKBB participants had a lower prevalence of both DKD (374/5570, 6.7%) and DR (355/5843, 6.1%) as compared to SEED (DKD prevalence: 555/2753, 20.2%, and DR prevalence: 685/2693, 25.4%), with only 0.7% (41/5570) having both complications. SEED had a median age of 61.7 (IQR 53.5-69.4) years and 49.1% (1361/2772) women, whereas UKBB had a median age of 61.0 (IQR 55.0-65.0) years and 35.8% (2090/5843) women. SEED was a multiethnic sample based in Singapore with 36.8% (1020/2772) Malay, 45.5% (1262/2772) Indian, and 17.7% (490/2772) Chinese individuals. In contrast, over 81.8% (4778/5843) of the UKBB participants were British, with the rest being Indian (217/5843, 3.7%), Irish (132/5843, 2.3%), Caribbean (93/5843, 1.6%), African (85/5843, 1.5%), or other ethnicities (538/5843, 9.2%).

Figure 2 shows the top 50 variables arranged in descending orders of their variable importance. For DKD detection, LASSO identified 15 variables with a frequency exceeding the cutoff, while GBDT identified 13, so we used the top 15 for deriving the corresponding DKD models; then for DR, LASSO identified 10 variables, while GBDT identified 6, so we used the top 10 for DR.

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In the internal validation, GBDT models performed the best with an AUC of 0.838 for DKD and 0.790 for DR, followed by LASSO with AUC values of 0.832 and 0.779, respectively. In contrast, LR only achieved AUC scores of 0.743 for DKD, and 0.764 for DR. In the external validation using UKBB data, LASSO models exhibited the best performance with AUC values of 0.791 for DKD, and 0.778 for DR. GBDT models achieved AUC scores of 0.738 and 0.778, respectively, while LR resulted in AUC values of 0.691 and 0.760 ( Figure 3 ).

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Further tests confirmed that the AUC scores of ML models were significantly higher than those obtained using traditional LR (2-sided P <.001). In terms of sensitivity and specificity at the optimal threshold (where sensitivity=specificity), LASSO and GBDT achieved comparable performance in internal validation (for DKD, 0.757 by LASSO vs 0.751 by GBDT; for DR, 0.708 by LASSO vs 0.709 by GBDT), and both were superior to LR (0.674 for DKD and 0.696 for DR). In external validation, LASSO performed the best, with 0.723 for DKD and 0.716 for DR. At 0.8 sensitivity, LASSO achieved 0.636 specificity for DKD detection in UKBB, and 0.617 for DR, outperforming the other 2 models ( Table 1 ).

a AUC: area under the receiver operating characteristic curve.

b SN: sensitivity.

c SP: specificity.

d Final sample size after variable selection and missing data removal.

e DKD: diabetic kidney disease.

f SEED: Singapore Epidemiology of Eye Diseases.

g LR: logistic regression.

h LASSO: Least Absolute Shrinkage and Selection Operator.

i GBDT: gradient-boosting decision tree.

j UKBB: UK biobank.

k DR: diabetic retinopathy.

In the sensitivity analysis, we evaluated the potential improvement in AUC by introducing additional variables in nested models ( Multimedia Appendix 5 ). The results supported the effectiveness of using the top 10 variables for detecting DR in SEED. In line with this, using all 239 variables did not improve beyond what was achieved with the top 10 in both internal validation (LASSO 0.776 and GBDT 0.783) and external validation (LASSO 0.754 and GBDT 0.774). Yet for DKD, incorporating additional variables beyond the top 15 resulted in improved AUC in SEED. Specifically, using all 239 variables yielded higher AUC values of 0.859 for LASSO and 0.842 for GBDT. However, this increase may be attributed to overfitting, as a similar pattern was not observed in external validation. Further, using all available variables resulted in lower AUC values for both LASSO (0.694) and GBDT (0.721) in the UKBB cohort. Consequently, we decided to maintain the DKD model with the top 15 variables for its simplicity and effectiveness.

For DKD, both LASSO and GBDT selected 5 risk factors (duration of diabetes, history of CVD, antihypertensive medication use, age, and insulin use) and 4 metabolites (tyrosine, lactate, cholesterol esters to total lipid ratio in intermediate-density lipoprotein particles [IDL-CE%], and citrate). For any DR, both algorithms identified 6 risk factors (insulin use, HbA 1c %, duration of diabetes, random blood glucose, age, and PP) and 2 metabolites (tyrosine and alanine). In the supplementary analysis, tyrosine was again selected for moderate or worse DR detection ( Multimedia Appendix 6 ).

Table 2 shows the association of the machine-learned metabolites with DKD and DR in multivariable LR models. We found tyrosine to be negatively associated with both DKD (OR 0.65, 95% CI 0.58-0.73; P <.001) and DR (OR 0.90, 95% CI 0.81-1.00; P =.047). High levels of alanine were associated with increased DR prevalence (OR 1.31, 95% CI 1.18-1.45; P <.001), but with decreased DKD prevalence (OR 0.72, 95% CI 0.64-0.80; P <.001). Similarly, a high level of lactate was linked to higher DR prevalence (OR 1.16, 95% CI 1.05-1.28; P =.004) but with lower DKD prevalence (OR 0.71, 95% CI 0.63-0.80; P <.001). Finally, a high level of citrate was associated with increased DKD prevalence (OR 1.90, 95% CI 1.70-2.12; P <.001), while high IDL-CE% was linked to decreased DKD prevalence (OR 0.46, 95% CI 0.40-0.53; P <.001).

a DKD: diabetic kidney disease.

b DR: diabetic retinopathy.

c OR: odds ratio.

d IDL-CE%: cholesterol esters to total lipid ratio in intermediate-density lipoprotein particles.

Principal Findings

ML selected age, use of insulin, duration of diabetes, and circulating tyrosine as the most important markers for DKD and DR detection in the SEED population with diabetes. Additionally, DKD was associated with the use of antihypertensive medications, CVD history, and 3 metabolites (lactate, citrate, and IDL-CE%), whereas DR was additionally linked to HbA 1c , random blood glucose, PP, and alanine.

The ML models developed in the SEED cohort with diabetes were externally validated using UKBB data. In both cohorts, ML models outperformed the traditional LR in terms of AUC, sensitivity, and specificity, demonstrating their potential to discover novel biomarkers and enable disease screening when integrated with health care and metabolite data.

Comparison With Prior Work

Our main data set included a comprehensive set of 19 risk factors and 220 circulating metabolites measured in 2772 individuals. The detailed patient profiling with a robust sample size allowed an opportunity to identify the markers most relevant to DKD and DR, offering insights into the systematic alteration of metabolism and underlying pathways.

In line with the previous literature [ 4 , 13 ], ML consistently identified 3 key factors—diabetes duration, age, and the use of insulin—as the top risk factors for both DKD and DR. However, ML also revealed novel aspects concerning some established risk factors for these conditions. For instance, our ML models exhibited a notable preference for PP over SBP and DBP in DR detection, supporting the study by Yamamoto et al [ 21 ] that PP, as a surrogate marker of arterial stiffness, reflected both the SBP elevation and DBP reduction, thereby carrying more predictive information for DR than other blood pressure metrics. Another example was assessing the relative importance of glycemia control indicators, where HbA 1c % consistently received a higher selection frequency in our ML models compared to random blood glucose levels. This was probably because HbA 1c % was averaged to reflect a mean shift with much less random noise, while random blood glucose data might carry more noise from life cycle changes and interindividual variability [ 22 ]. Interestingly, some well-established risk factors usually included such as sex did not appear in the top-ranking lists by ML, although this variable had been selected by traditional LR models on the same population in previous studies [ 23 ]. This could be because sex is an intrinsic component of other phenotypes. For instance, male sex was associated with CVD [ 24 ], a condition well-known to be linked to DKD and DR [ 2 ].

We noted high levels of tyrosine, an aromatic amino acid, to be negatively associated with the prevalence of DKD and DR, supporting the ADVANCE trial where increased tyrosine concentration was linked to a decreased risk of diabetic microvascular events (hazard ratio 0.78, 95% CI 0.67-0.91) [ 12 ]. Tyrosine is mainly synthesized from phenylalanine hydroxylation in the liver and kidney; impaired kidney function is therefore associated with reduced phenylalanine hydroxylase activity characterized by low blood tyrosine levels [ 12 ]. Additionally, tyrosine serves as a precursor to catecholamine neurotransmitters (dopamine, norepinephrine, and epinephrine), and plays a pivotal role in central nervous system functions and activities [ 25 ]. In metabolic disorders such as diabetes, reduced blood tyrosine can affect its uptake into the brain and also the synthesis and release of transmitters, thereby altering hormonal function, affective state, and blood pressure [ 25 ], potentially linked to higher microvascular risks.

Another amino acid selected by ML was alanine, and its higher concentration in blood was associated with higher DR prevalence, but lower DKD prevalence. In the ADVANCE trial, a negative association was reported between circulating alanine and an aggregated microvascular outcome, defined as new or worsening nephropathy or retinopathy (hazard ratio 0.86, 95% CI 0.76-0.98) [ 12 ]. In other research, high levels of blood amino acids such as alanine have been linked to inhibited insulin signaling to glucose transport, phosphorylation, and glycogen synthesis, causing insulin resistance, which impairs hepatic mitochondrial function in patients with diabetes and contributes to diabetes microvascular complications such as DR [ 26 , 27 ].

Among the ML-selected metabolites for DKD, high levels of lactate were found to be associated with lower DKD prevalence. However, another study conducted in the DKD population suggested an accumulation of acid due to abnormal lactate metabolism causing fibrosis and mitochondrial abnormalities, leading to further kidney damage [ 28 ]. Nevertheless, further investigations are required to understand the complex association between circulating lactate and DKD. Another metabolite identified was citrate, which showed a positive association with DKD in SEED. In people with DKD, an elevated plasma citrate level has been linked to reduced organic anion transport and dysregulated mitochondrial functions of the kidney tissues [ 8 ]. Our study also highlighted the significance of IDL-CE% in diabetic microvascular complications, revealing its inverse association with prevalent DKD in the SEED population with diabetes. Aberrations in lipoprotein composition have been reported to be indicative of insulin resistance and impaired glucose tolerance in the general population, known to cause future diabetes [ 9 , 27 ]. In people with type 1 DKD, abnormality in lipoproteins clearance was further linked to impaired kidney function [ 29 ].

For biomarker discovery, traditional studies often relied on LR models to examine metabolites one by one separately [ 12 , 30 ], with stringent model assumptions and multiple testing corrections [ 9 , 31 ]. Herein, our ML approach was more efficient in the sense that it simultaneously examines all variables for potential associations. While LASSO was limited to detecting linear associations, we implemented GBDT as a complementary model to additionally assess nonlinear terms and high-order interactions. As was shown in Figure 2 , DR-related variables in GBDT had higher selection frequencies than in LASSO, suggesting the existence of such complex associations. Still, LASSO models achieved superior performance in external validation, indicating a prominent contribution of linear associations to DKD and DR detection. Another highlight of our methodology was the repeated cross-validation, which reduced the influence of potential outliers and ensured the randomness of sampling, thereby generating results more robust than those relying on a fixed training set. This approach also allowed us to quantify individual contributions of variables based on their selection frequencies. However, alternative definitions of variable importance scores [ 32 ] may be considered in a future study to potentially refine the variable selection process.

Globally, three-quarters of those with diabetes live in low- and middle-income countries, particularly India and China [ 33 ]. While the risk profile of Asian patients with diabetes differs from that in high-income “Western” societies in terms of age, BMI, lifestyle, diet, and many other aspects [ 33 ], there has been limited discussion on Asian populations with diabetes. Our study, conducted in Asian populations (Malay, Indian, and Chinese), may contribute to the topic by allowing an opportunity to identify the commonalities and differences between DKD and DR in terms of circulating metabolic traits, offering insights into the systematic alteration of metabolism in diabetes.

Limitations

In total, 1 limitation of this study was that we did not separate study subjects by diabetes type. Since around 95% of the SEED population with diabetes had type 2 diabetes, our results would mainly reflect the variable associations with type 2 diabetes. While these associations hold potential for hypothesis generation and disease detection, the cross-sectional nature of our study highlights the need for caution in drawing causal inferences. Future longitudinal studies are warranted to establish temporal associations based on our current findings.

Another issue was data availability—albuminuria, an important indicator of kidney disease [ 13 ], and 3 metabolites (pyruvate, glycerol, and glutamine) were missing in Malay individuals of the SEED population. Hence, these variables were excluded from the analysis. Certain diabetic medications were reported to potentially interfere with circulating metabolite levels [ 12 , 34 , 35 ]. However, we could only account for the use of insulin due to limited data availability on UKBB medication profiles. Furthermore, we noted a difference in defining DR between our development data set (SEED), which used the standard ETDRS classification system, and the external test set (UKBB), where DR cases were identified using ICD-10 codes. This variance in outcome definition might have introduced some degree of misclassification in UKBB. Additionally, due to the absence of ETDRS-based severity scores in UKBB, validation of the supplementary model for moderate or worse DR in supplementary analysis was not feasible. Nevertheless, it was noteworthy that the features identified in SEED remained relevant for enhancing DR detection in the UKBB data set. However, we acknowledge that the discrepancy in DR definition underscores the need for caution when interpreting results, particularly in the context of clinical or practical applications. Future research may benefit from adopting a uniform approach to DR classification to minimize such limitations and enhance result validity.

Conclusions

In conclusion, current ML models developed using the SEED population with diabetes and subsequently validated in UKBB showed superior performance compared to traditional LR for DKD and DR detection. ML highlighted age, use of insulin, diabetes duration, and tyrosine as the most influential factors in DKD and DR. Additionally, DKD was associated with high levels of citrate, low levels of lactate, and low IDL-CE%; while DR was linked to higher levels of alanine. The integration of ML with health care data and metabolomics could facilitate biomarker selection and enable disease screening.

Acknowledgments

We thank all the participants of Singapore Epidemiology of Eye Diseases (SEED) and UK biobank (UKBB) for contributing their health profiles to support our research. ChatGPT (OpenAI) [ 36 ] was used solely for proofreading purposes, focusing on grammar and spelling corrections. All such revisions were reviewed and validated by human coauthors. This study was supported by the Singapore Ministry of Health’s National Medical Research Council (NMRC/StaR/016/2013, NMRC/CIRG/1371/2013, NMRC/CIRG/1417/2015, and OFLCG/001/2017). The funders had no role in study design; data collection, management, analysis, and interpretation; preparation, and review of this paper; or decision to publish.

Data Availability

As the Singapore Epidemiology of Eye Diseases (SEED) study involves human participants, the data cannot be made freely available in this paper, the supplemental files, or a public repository due to ethical restrictions. Nevertheless, the data are available from the Singapore Eye Research Institutional Ethics Committee for researchers who meet the criteria for access to confidential data. Interested researchers can send data access requests to the Singapore Eye Research Institute. The UK biobank (UKBB) test data set was obtained from the UKBB (application 45925). Data cannot be shared publicly due to the violation of patient privacy and the absence of informed consent for data sharing.

Authors' Contributions

All authors contributed to the intellectual development of this paper. JL and CS designed this study and supervised data analysis. FH and CNYL wrote the initial draft. FH performed the statistical analyses. SN, CC, TYW, JL, and CS assisted in the interpretation of the analyzed data and provided critical corrections to this paper. CS is the guarantor of this work and as such had full access to all the data in this study and takes responsibility for the integrity of the data and accuracy of the data analysis. The final version of this paper has been seen and approved by all the authors.

Conflicts of Interest

None declared.

List of 239 Singapore Epidemiology of Eye Diseases (SEED) variables included for machine learning feature selection.

Distribution of missing data in Singapore Epidemiology of Eye Diseases (SEED), column-wise and row-wise histograms.

Population characteristics of Singapore Epidemiology of Eye Diseases (SEED) stratified by diabetic kidney disease (DKD) and diabetic retinopathy (DR) status.

Comparative analysis of population characteristics: Singapore Epidemiology of Eye Diseases (SEED) vs UK biobank.

Area under the receiver operating characteristic curve performance of nested models in internal validation with varying numbers of machine-learned variables.

Top-50 machine-learned variables for moderate or worse diabetic retinopathy (DR) detection.

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Abbreviations

Edited by A Mavragani; submitted 18.01.23; peer-reviewed by KH Hung, G Lim; comments to author 25.09.23; revised version received 12.10.23; accepted 19.12.23; published 28.03.24.

©Feng He, Clarissa Ng Yin Ling, Simon Nusinovici, Ching-Yu Cheng, Tien Yin Wong, Jialiang Li, Charumathi Sabanayagam. Originally published in the Journal of Medical Internet Research (https://www.jmir.org), 28.03.2024.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in the Journal of Medical Internet Research, is properly cited. The complete bibliographic information, a link to the original publication on https://www.jmir.org/, as well as this copyright and license information must be included.

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    Conclusion Sections in Scientific Research Reports (IMRaD) In IMRaD* reports, conclusions often fall under the discussion section. In some disciplines and journals, however, conclusions are separated from discussions. If this is the case for the paper you are working on, you may find the following description of common conclusion moves and ...

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